In-vitro Response of Gladiolus (var. American Beauty) towards Plant Growth Regulators Using Cormel Explants

Khwairakpam Lily Devi

Department of Horticulture, College of Agriculture (CAU), Pasighat, Arunachal Pradesh, India.

Soumen Maitra

Department of Floriculture and Landscape Architecture, Uttar Banga Krishi Viswavidyalaya, Pundibari, Cooch Behar, West Bengal, India.

Manas Mandal *

Department of Floriculture and Landscape Architecture, Uttar Banga Krishi Viswavidyalaya, Pundibari, Cooch Behar, West Bengal, India.

Rocky Thokchom

Department of Agriculture, South Asian Institute of Rural and Agricultural Management, Manipur, India.

*Author to whom correspondence should be addressed.


Gladiolus is one of the most potential bulbous cut flower crops cultivated widely in India due to its attractive and long lasting spikes. The plant is propagated vegetatively through corms and cormels, but due to low rate of multiplication, its cultivation is hindered. Also, it is grown through underground modified stems, they are more often attacked by soil borne diseases such as Fusarium corm rot. In-vitro propagation techniques, assumes significance, especially for securing rapid multiplication of quality planting propagules using dormant cormlet explants and media. The results of the study revealed that the MS growing medium supplemented with 2 mg/l BAP showed improved results in respect of sprouting of explants (54.46 %), days required for sprouting of explants (8.68 DAI) and shoot length at 30 (6.32 cm), 60 (12.40 cm) and 90 (13.21 cm) DAI. The in vitro regenerated gladiolus plantlets when placed in the rooting MS medium supplemented with 4 mg/l IBA showed earlier root initiation (8.31 DAI) and higher number of roots/shoot (5.37 DAI) as compared to the rest of the treatment combinations. Hardening medium consisting of garden soil + sand + vermicompost (1:1:1) showed greater survivability (50.98 %) of plantlets. Thus, the study has been initiated an efficient protocol for in-vitro propagation of gladiolus through cormlets cultured in MS media containing BAP and IBA as shooting and rooting plant growth regulators and subsequent acclimatization in garden soil + sand+ vermicompost medium.

Keywords: Gladiolus, cormels, growth regulators, in-vitro, quality planting

How to Cite

Devi , K. L., Maitra , S., Mandal , M., & Thokchom , R. (2023). In-vitro Response of Gladiolus (var. American Beauty) towards Plant Growth Regulators Using Cormel Explants . International Journal of Environment and Climate Change, 13(7), 388–395.


Download data is not yet available.


Singh KA, Kumar A, Ghimire R. Performance of Indian and exotic varieties of gladiolus under eastern Uttar Pradesh conditions. The Asian Journal of Horticulture. 2013;1(8):191-194.

Safeena SA, Thangam M, Devi SP, Desai AR, Arunachalam V, Gupta MJ, Singh NP. Ready reckoner on cultivation of gladiolus. Technical Bulletin No. 43. ICAR Research Complex for Goa (Indian Council of Agricultural Research) Ela Old Goa -403402 Goa India; 2014.

Nasir I. A, Jamal A, Tabassum B, Husnain T. Regeneration response from old cell suspension cultures of gladiolus. Experimental Agriculture & Horticulture. 2013;2:1-11.

Memon NN, Wahocho Miano FT, Leghari HM. Propagation of gladiolus corms and cormels: A review. African Journal of Biotechnology. (016;15(32)1699- 1710.

Misra RL. Propagation of gladiolus through sprouts- An entirely new method in gladiolus propagation. In: Prakash J and Bhandary K R. (Ed.):Floriculture- Technology trades and trends. Oxford & IBH Pub.Co. Pvt. Ltd. India. 1994;67-70.

Memon NUN, Qasim M, Jaskani M. Pakisthan Journal of Agriculture Science. 2010;47(2):115-123

Jala A. Potential of benzyl adenine naphthalene acetic acid and sucrose concentration on growth development and regeneration of new shoot and cormel on gladiolus. Scientia Horticulturae. 2013; 12(4):277-285.

Murashige T, Skoog F. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum. 1962;15:473-497.

Roy KS, Gangopadhyay G, Bandhopadhyay T, Modok KB. Enhancement of in vitro micro corm production in Gladiolus using alternative matrix. African Journal Biotechnology. 2009; 12(3):202-206.

Beura S, Singh R, Jagadev PN. In-vitro multiplication studies in gladiolus cv. American beauty. Orissa Journal of Horticulture. 2003;31(1):101-105.

Parisa K, Ashkaneh K, Sameneh K, Mohammad FGB. Tissue Culture Process Adaptation and Maturation of Gladiolus Corms in order to Produce Fusarium Resistant Corms by Genetic Engineering. Journal of Biosafty. 2021;14(2):101-116.

Rodríguez-Gacio M. D. C, Matilla-Vázquez M. A. and Matilla A. J. (2009). Seed dormancy and ABA signalling: The breakthrough goes on. Plant Signaling and Behavior 4(11):1035-1048.

Hussey G. In-vitro propagation of gladiolus by precocious axillary shoot formation. Sci. Hortic. 1977;6:287-296.

Silva T, Gulyas A, Tabori MK, Wang CQ, Wang RM. In vitro tissue culture of apple and other Malus species: recent advances and applications. Springer. 2019;249:975-1006.

Mateen RM. Development and optimization of micro- propagation in-vitro methodology for Gladiolus. Bio Scientific Review. 2019;1(2):21-36.

Budiarto K. In-vitro regeneration of three gladiolus cultivars using cormel explants. Jurnal ILMU DASAR. 2009;10(2):109-113.

Maitra S, Roychowdhury N, Ghosh PD, Satya P. Towards development of a suitable protocol for regeneration of gladiolus (Gladiolus grandiflorus L.) in-vitro. International Journal of Bio-resource and Stress Management. 2011;2(3):283-286.

Situma MN, Mwangi M, Mulwa RMS. Effects of benzyl adenine and gibberellic acid pre-treatments on dormancy release flowering time and multiplication of oriental lily (Lilium longiflorum) bulbs. Journal of Applied Horticulture. 2015;17(1):26-30.

Sajjad Y, Jaskani MJ, Mehmood A, Qasim M, Akhtar G. Alleviation of gladiolus (Gladiolus grandiflorus) corm dormancy through application of 6-benzylaminopurine and gibberellic acid. Pakistan Journal of Botany. 2020;52(3):1-8.

Kumar A, Kumar A, Sharma V, Mishra A Singh S, Kumar P. In-vitro Regeneration of Gladiolus (Gladiolus hybrida L.): Optimization of Growth Media and Assessment of Genetic Fidelity. International Journal of Current Microbiology and Applied Sciences. 2018;7(10):2900-2909.

Priyakumari I, Sheela VL. Micropropagation of gladiolus cv. ‘Peach Blossom’ through enhanced release of axillary buds. Journal of Tropical Agriculture. 2005;43(1-2):47-50.

Torabi-Giglou M, Hajieghrari B. In-vitro study on regeneration of Gladiolus grandiflorus corm calli as affected by plant growth regulators. Pakistan Journal of Biological Sciences. 2008;11(8):1147-1151.

Pragya, Singh SK, Misra RL, Ranjan JK. In-vitro shoot regeneration from cormel derived callus of gladiolus and bio-hardening of plantlets. Indian Journal of Biotechnology. 2012;11(1):99-104.

Haouala F, Salhi I. Axillary budding and rooting of gladiolus (Gladiolus grandifloras Hort.) in salt stress conditions. Afr. J. Hort. Sci. 2012;6:101-110.

Aslam J, Mujib A, Sharma MP. In-vitro micropropagation of Dracaena sanderiana Sander ex Mast: An important indoor ornamental plant. Saudi Journal of Biological Sciences. 2013;20:63–68.

Chaidaroon S, Ungvichian I, Ratanathavornkiti K. In-vitro root initiation of ‘Champi Sirindhorn’ (Magnolia sirindhorniae Noot. & Chalermglin). Assumption University Journal of Technology. 2004;129-132.

Patil MS, Adiga JD, Reddy BS, Patil BR. In-vitro multiplication studies in gladiolus cv. American Beauty. Karnataka Journal of Horticulture. 2004;1(1):48-51.

Maitra S, Roychowdhury N, Ghosh PD, Satya P. Effect of kinetin and NAA on regeneration of carnation (Dianthus caryophyllus L.) from shoot-tip explants. International Journal of Bio-resource and Stress Management. 2011;2(3):287-292.

Salih EE, AlSalihy AA. Using of UV light Type C and growth regulators in propagation and cormels formation in Gladiolus Spp. Annals of R.S.C.B. 2021;25(4):8486 – 8497.

Rabindra MB, Thomas P. Protocol for hardening of in-vitro raised grape plantlets. In: Mukhopadhyay S. (Ed.): Commercialization of micropropagated plants in India. Naya Prokash Calcutta India. 1995;72.

Aslam F, Naz S, Tariq A, Ilyas S, Shahzadi K. Rapid multiplication of ornamental bulbous plants of lilium orientalis and lilium longiflorum. Pakistan Journal of Botany. 2013;45(6):2051-2055.

Singh P, Jyoti B, Ahmad T. Gladiolus- queen of bulbous flowers. Rashtriya Krishi. 2017; 1(12):67-69.