Influence of Different Temperature Regimes and Culture Media on the Growth and Sporulation of Alternaria brassicae Causing Alternaria Blight of Mustard
Hemalatha S.
Department of Plant Protection, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh-202002, India.
R. U. Khan
Department of Plant Protection, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh-202002, India.
Sibte Sayyeda
Department of Plant Protection, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh-202002, India.
Vaibhav Pratap Singh *
Department of Plant Protection, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh-202002, India.
*Author to whom correspondence should be addressed.
Abstract
Mustard (Brassica juncea L.) is the second most important oilseed commodity in India. Alternaria blight (Alternaria brassicae) is one of the devastating fungal pathogen, resulting in severe yield losses to the crop. The present study is conducted in-vitro to know the effect of different temperature regimes and various culture media on the mycelial growth and sporulation of A. brassicae. It is clearly evident from the results that all the tested temperature regimes and culture media showed variation in the colony diameter and other cultural characteristics. The temperature of 25°C significantly encouraged mycelial growth (85.00 mm) and exhibited excellent sporulation of A. brassicae compared to 15 and 35°C. In case of culture media, Potato Dextrose Agar was found to be significantly superior over the other media and registered maximum mycelial growth (82.00 mm) with excellent sporulation. However, Oat Meal Agar supported the minimum mycelial growth (45.00 mm) with fair sporulation. The variation in cultural characteristics (colony color, growth, appearance, and shape etc.) was also observed with various culture media.
Keywords: Temperature, culture media, rapeseed-mustard, A. brassicae, Alternaria blight
How to Cite
Downloads
References
Kumar A. Challenge of edible oils: Can Brassicas deliver. J Oilseed Brassica. 2014;5:83-86.
Yadav MS, Godika S, Yadava DK, Ahmad N, Mehta N, Bhatnagar K, Agrawal VK, Kumar A, Thomas L, Chattopadhyay C. Prioritizing components of package of integrated pest management in Indian mustard (Brassica juncea) in India for better economic benefit. Crop Protection. 2019;120:21-29.
Singh L, Bagri RK, Thakore BBL, Singh J, Sharma P. Effect of pH source on production of enzyme by Fusarium oxysporum f. sp. pisi. Annals of Plant Protection Sciences. 2011;11:388-390.
Agricultural Statistics at a Glance. Directorate of Economics and Statistics, Department of Agriculture and Farmers Welfare, Ministry of Agriculture and Farmers Welfare, Government of India; 2021.
Saharan GS, Mehta N, Sangwan MS. Diseases of oilseed crops. Indus Publication Co., New Delhi. 2005;643.
Kolte SJ. Diseases of annual edible oilseed crops, Rapeseed-mustard and Sesame Diseases. CRC Press Inc Boca Raton. 1985;2:135.
Meena PD, Awasthi RP, Chattopadhyay C, Kolte SJ, Kumar A. Altemaria blight: a chronic disease in Rapeseed-mustard. J. Oilseed Brassica. 2010;1:1-11.
Saharan GS, Mehta N, Meena PD, Dayal P. Alternaria diseases of crucifers: biology, ecology and disease management. Springer. 2016;17-51.
Saharan GS, Mehta N. Fungal Diseases of Rapeseed-Mustard. In: Diseases of Field Crops (Gupta, V. K. and Paul, Y. S. eds.). Indus Publishing Company, New Delhi. 2002;193-228.
Aneja JK, Agarwal A, Agnihotri A. Inter and intra-specific diversity in Alternaria species infecting oilseed Brassicas in India. Journal of Oilseed Brassica. 2014;5(2):102-117.
Gholve VM, Ghuge SB, Pawar SV. Effect of different culture media, temperature and pH on growth and sporulation of Alternaria carthami. The Indian Society of Oilseeds Research. 2017;34(4):256-258.
Taware MR, Gholve VM, Wagh SS, Kuldhar DP, Pawar DV, Chavan AA. Effect of different culture media, temperature, pH, carbon and nitrogen sources on mycelial growth and sporulation of Alternaria carthami causing Alternaria blight of safflower. International Journal of Plant Protection. 2014;7(2):349-353.
Yadav SP, Kumar S, Prasad R, Upadhyay H, Bansal M. Identification of morphological, cultural and pathogenic variability of Alternaria brassicae causing Alternaria blight of Indian mustard (Brassica juncea). Indian Phytopathology. 2016;69(1):102-104.
Panchal DG. Alternaria fruit rot of tomato (Lycopersicon esculentum Mill.) and its management. M. Sc. thesis Submitted to Anand Agricultural University, Anand; 2008.
Kurhade KC, Gangawane HD, Deokar CD, Raghuwanshi KS. Effect of temeperature and pH on growth of Alternaria tagetica, Leaf blight of marigold. Bhartiya Krishi Anusandhan Patrika 2021;36(1):76-78.
Charith P, Mishra J P, Prasad R, Chandra J, Sekhar VPR, Kumar S. Favorable cultural conditions for mycelial growth of Alternaria brassicae, causing leaf blight of cabbage under in-vitro conditions. Journal of Pharmacognosy and Phytochemistry. 2020;9(4):831-834.
Nagrale DT, Gaikwad AP, Sharma L. Morphological and cultural characterization of Alternaria alternata (Fr.) Keissler blight of gerbera (Gerbera jamesonii H. Bolus ex J D Hook). Journal of Applied and Natural Science. 2013;5(1):171-178.
Meena PD, Gour RB, Gupta JC, Singh HK, Awasthi RP, Netam RS, Godika S, Sandhu PS, Prasad R, Rathi AS, Rai D, Thomas L, Patel GA, Chattopadhyay C. Non-chemical agents provide tenable, eco-friendly alternatives for the management of the major diseases devastating Indian mustard (Brassica juncea) in India. Crop Protection. 2013;53:169-174.
Koley S, Mahapatra SS. Evaluation of culture media for growth characteristics of Alternaria solani, causing early blight of tomato. Journal of Plant Pathology and Microbiology. 2015;s1. DOI:10.4172/2157-7471.S1-005.
Krishna VP, Suryawanshi AP, Prajapati S, Surekha S. Effect of different culture media on growth and sporulation of Alternaria dauci causing carrot leaf blight. Journal of Pharmacognosy and Phytochemistry. 2018;7(6):1789-1792.
Reddy VV, Ghante PH, Kanase KM. Studies on morpho-cultural characters of Alternaria alternata infecting groundnut crop by using various culture media. Journal of Pharmacognosy and Phytochemistry. 2019;8(2):85-87.
Savitha AS, Naik MK, Kumar AK. Cultural, Morphological, Physiological and Pathogenic Diversity among the Isolates of Alternaria sp. Incitant of Blight Disease of sesame. Journal of Mycopathological Research. 2013;51:259-265.
Hubballi M, Nakkeran, S., Raghuchander T, Smyappan R. Growth of Alternaria alternata causing leaf blight of noni on various culture media. World J. Agric. Sci. 2010;6(2):171-177.
Khan MM, Khan RU, Mohiddin FA. In vitro effect of temperature and culture media on the growth of A. brassicae infecting rapeseed-mustard. Annals of Plant Protection Sciences. 2007;15(2): 526-527.
Yadhav R, Khan RU. Differential response of Alternaria brassicicola isolates at different temperatures and nutrient media. J. Eco. Agric. 2008;3(1):72-74.
Singh VP, Khan RU. Effect of different nutrient media on the radial growth of Alternaria brassicae (Berk.) Sacc. infecting different Brassica host crops. International Journal of Agricultural invention. 2018; 3(2):137-140.