Characterization of Chemical Tests in Different Indica Rice Accessions and Power of Discriminating Rice Subvarieties in North Western Himalayas

Asha Kumari *

ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand-263601, India.

Chandan Maharana

ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand-263601, India.

Jay Prakash Aditya

ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand-263601, India.

Lakshmi Kant

ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand-263601, India.

*Author to whom correspondence should be addressed.


Variety identification has great significance from seed production, breeding as well as intellectual property rights point of view to ensure quality seed. The decrease in the morphological variation among the major rice varieties due to narrow genetic base makes it necessary for the development of quick and reliable tests for varietal identification particularly for those working in seed certification and quality maintenance. The study was conducted during 2019-20 at the laboratories of the Crop Improvement Division, ICAR-VPKAS, Almora. Two hundred Indica rice varieties/genotypes were identified on the basis of seed color (phenol, modified phenol, NaOH and KOH) to chemical tests. Phenol test grouped variety into three distinct groups viz., dark brown (15 varieties), brown (59 varieties) and light brown (95 varieties). With the help of modified phenol test these varieties were further sub grouped. NaOH test grouped varieties into two distinct groups i.e., light yellow (177 varieties) and wine red (23 varieties). These tests clearly differentiated the varieties of one group to that of another groups on the basis of seed coat color. However, KOH test was not reliable for the differentiation of these varieties because all these varieties showed light yellow color after treating with KOH solution. Though no individual chemical test was able to distinguish all the varieties, different chemical tests in conjunction were useful in identification of varieties. Thus these simple, reliable and quick tests can be used for varietal identification in rice crop.

Keywords: Rice varieties, chemical tests, phenotype, seed color

How to Cite

Kumari , Asha, Chandan Maharana, Jay Prakash Aditya, and Lakshmi Kant. 2023. “Characterization of Chemical Tests in Different Indica Rice Accessions and Power of Discriminating Rice Subvarieties in North Western Himalayas”. International Journal of Environment and Climate Change 13 (8):1246-54.


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Vijayalakshmi B, Vijay D. Development of seed keys for varietal identification in Rice (Oryza sativa L.) using chemical tests. Seed Res. 2009;37(1&2):56-61.

Tiwari JK, Rastogi NK, Chandrakar PK, Sarawagi AK, Verulka SB. Identification of rice varieties through chemical tests . Seed Research. 2013;41(1): 83-90.

Vishwanath K, Pallavi HM, Nethra N, Rajendra Prasad S. Chemical Tests for Identification and Characterization of Tomato Cultivars. Plant Breeding and Seed Science. 2013;67:4-8.

Arus P, Tanksley SD, Oroton TJ, Jones RA. Electrophoresis variation as a tool for determining seed purity and for breeding hybrid varieties of Brassica oleraceae. Euphytica. 1982;31:417-428.

Tanksley SD, Ganal SD, Prince JP, Broun P, Roder MS, Young ND. High density molecular maps of the tomato and potato genomes. Genetics. 1992;132:1141-1460.

Lucchese C, Dinelli G, Miggiano A, Lovato. Identification of pepper (Capsicum spp) cultivars by field and electrophoresis tests. Seed Science and Technology. 1999;27: 37-47.

Vishwanath K, Ananthararayanan TV, Pallavi HM, Ramegowda, Prasad S, Rajendra, Prassana KPR, Shailaj H. Varietal Characterization of Tomato Cultivars Based on RAPD Markers. Research Journal Agriculture Biological Sciences. 2010;6(6):713-715.

Papp E, Korosi F, Kemeny A. Identification of seeds of Brassica species on the basis of spectrophotometry of seed extracts. Seed Science & Technology. 1997;25: 577-580.

Vanangamudi K, Palamisamy V, Natesan P. Variety determination in rice phenol and potassium hydroxide tests. Seed Science Technology. 1988;16:465-470.

MC Donald, MB JR. AOSA cultivar purity subcommittee report. AOSA Newsletter. 1985;59(1):40-57.

Punia RC, Chandgi R, Tomer RPS, Anita M. Cultivar identification of wheat by modified phenol tests. Seed Technology News. 2002;32 (1) 97.

Patil BNK, Macha Sangeeta, Motagi BN, Vijaykumar AG, Hanchinal RR. Characterization of safflower varieties through chemical tests. Proceedings of XII National Seed Seminar on prosperity through quality seed, ANGRAU, Hyderabad, India. 2006;168.

Nethra N, Rajendra Prasad S, Vishwanath K, Dhanraj KN, Gowda R. Identification of rice hybrids and their parental lines based on seed, seedling characters, chemical tests and gel electrophoresis of Total soluble proteins. Seed Science & Technology. 2007;35:176-186.

Walls FW. A standardized phenol method for testing wheat for varietal purity. Handbook on seed testing, AOSA. 1965;28.

Banerjee SK, Chandra R. Modified phenol test for varietal identification of wheat. Seed Science Technology. 1977;5:53-66.

Chakrabarty SK, Agrawal RL. Identification of blackgram varieties II. Utilization of morphological characteristics of seedlings. Seed Research. 1989;17(2):139- 142.

Joshi MG, Banerjee SK. Genetics of phenol colour reaction in emmer wheats. Proceedings of International Seed Testing Association. 1970;35:207.

Singh C, Jeevan Kumar SP, Sripathy KV, Soma Sundram G, Udaya Bhaskar K, Ramesh KV, Kumar M, Rajendra Prasad S. Characterization and Identification of Rice Germplasm Accessions Using Chemical Tests. Seed Research. 2017; 45(1):75-83.

Gupta N, Monika J, Sarao A, Navraj K,Sharma RC. Utility of phenol test in varietal characterization. Crop Improvement. 2007;34 (1): 77-81.

Anita lakshmi V, Gowda R, Sathisha CS, Prasad Rajendra. Varietal response to various chemical tests for their characterization in rice. Indian Journal Plant Sciences. 2014;3(2):177- 179.

Masuthi D, Vyakarnahal BS, Deshpande VK, Jagadeesha RC, Chavan Mukesh L, Rubina K. Characterization of traditional aromatic rice cultivars by chemical markers. International Journal of Advance Research. 2015;3(5):103- 107.

Dileep Kumar M, Vyakarnahal BS, Deshpande RC, Jagdeesha, Mukesh LC, Khaja Rubina S.. Characterization of traditional aromatic rice cultivars by chemical markers. International Journal of Advance Research. 2015;3(5):103-107.

Vijaylakshmi B, Vijay D. Development of Seed Keys for Varietal Identification in Rice (Oryza sativa L.) using Chemical Tests. Seed Research. 2009;37(1&2): 56-61.

Gupta PK, Agarwal RK. Determination of varietal purity of paddy varieties by laboratory evaluation. Oryza. 1987;25: 310- 314.

Nagaraju KS, Prasanna KPR, Rajendra Prasad S, Mohan Rao A, Vishwanath K. Identification of chilli (Capsicum Annuum L.) cultivars based on chemical tests and SDS-page of total soluble proteins. International Journal of Chemical Studies. 2020;8(5):2128-2133.

Rai H, Peerzada Ovais Hamid, Dahiya OS, Jakhar SS. Varietal Identification Based on Chemical Methods in Different Varieties of Indian Mustard (Brassica juncea (L.) Czern. & Coss.). International Journal of Current Microbiology and Applied Sciences. 2019;8(5): 2391-2396.

Saharan V. Varietal identification in rice. M.Sc. thesis, Haryana Agricultural University, Hisar, India; 1991.