Improvement of Safflower (Carthamus tinctorius L.) for Salinity Tolerance under in vitro Condition

S. D. Surbhaiyya *

Department of Plant Biotech, K. K. Wagh College of Agricultural Biotechnology, Nashik - 422003, India.

D. R. Dhumale

Department of Plant Biotech, K. K. Wagh College of Agricultural Biotechnology, Nashik - 422003, India.

M. S. Dudhare

Department of Animal Biotech, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani - 431402, India.

P. V. Jadhav

Biotechnology Centre, Department of Agricultural Botany (Agricultural Biotechnology), Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Maharashtra - 444104, India.

M. P. Moharil

*Author to whom correspondence should be addressed.


Abstract

Aim: The primary aim of the present study was to screen the salt tolerant calli and optimization of   in-vitro regeneration protocol from selected screen calli.

Methodology: The cotyledonary leaf explants was sterilized by using 1% Bavistin, 0.1% Mercuric chloride and 70% ethanol followed by washing with distilled water. Sterilized explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentration of NaCl (i.e. 0, 50mM, 100mM, 200mM, 300 mM and 400 mM) to check the salinity tolerance ability of PKV pink genotype. In vitro screening of callus was studied by morphological characters like colour and texture of callus, callus growth percentage, relative water contained, cell survivility and proline content from saline stress and unstress calli to confirm the saline tolerance pressure to regenerate the PKV Pink safflower genotype.

Results: MS medium supplemented with 150 mM NaCl showed 50% survival of calli, whereas no growth was obtained in high concentration of NaCl. Moreover, biochemical assay like proline estimation was done for its confirmation from different NaCl stress and unstress calli. The proline accumulation found to be highest callus grown on MS media supplemented with 150 mM NaCl as compared to control. Also studied the morphological observation i.e. colour and texture of calli, callus growth percentage, relative water content and cell viability under different NaCl stress to select the saline tolerance pressure to regenerate the tolerance line in PKV Pink. The saline tolerance shoots failed to produce in vitro rooting on the standardized rooting medium. So, the different approach like higher auxin shock and grafting experiment were attempted to overcome the rooting problem. Higher auxin shock experiment failed but grafting approach found satisfactory to overcome the rooting in saline tolerance shoot and for development of saline tolerance line in PKV Pink safflower genotype.

Conclusion: The development of abiotic stress tolerance plants found the better understanding of physiological and biochemical changes in plants under in vitro stress conditions. 

Keywords: Safflower, regeneration, salinity tolerance, screening


How to Cite

Surbhaiyya , S. D., Dhumale, D. R., Dudhare , M. S., Jadhav , P. V., & Moharil , M. P. (2023). Improvement of Safflower (Carthamus tinctorius L.) for Salinity Tolerance under in vitro Condition. International Journal of Environment and Climate Change, 13(8), 1978–1989. https://doi.org/10.9734/ijecc/2023/v13i82155

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References

Hasegawa PM. Sodium (Na+) homeostasis and salt tolerance of plants. Environmental and Experimental Botany. 2013;92:19‐31. Available:https://doi.org/10.1016/j.envexpbot.2013.03.001

Erdal SC, Cakirlar H. Impact of salt stress on photosystem II efficiency and antioxidant enzyme activities of safflower (Carthamus tinctorius L.) cultivars. Turkish Journal of Biology. 2014;38(4):549-560. Available:https://doi.org/10.3906/biy-1401-33

Verma S, Mishra SN. Putrescine alleviation of growth in salt stressed Brassica juncea by inducing antioxidative defense system. Journal of Plant Physiology. 2005;162(6):669-677. Available:https://doi.org/10.1016/j.jplph.2004.08.008

Merati MJ, Hassanpour H, Niknam V, Mirmasoumi M. Exogenous application of penconazole regulates plant growth and antioxidative responses in salt-stressed Mentha pulegium L. Journal of Plant Interaction.2014;9:791-801.

Available:https://doi.org/10.1080/17429145.2014.948084

Sorahinobar M, Niknam V, Ebrahimzadeh H, Soltanloo H, Behmanesh M, Enferadi ST. Central role of salicylic acid in resistance of wheat against Fusarium graminearum. Journal of Plant Growth Regulation.2016;35:477-491.

Available:https://doi.org/10.1007/s00344-015-9554-1

Rasheed R, Wahid A, Ashraf M, Basra SMA. Role of proline and glycinebetaine in improving chilling stress tolerance in sugarcane buds at sprouting. International Journal of Agriculture and Biology.2010;12(1):1-8.

Lutts S, Almansouri M, Kinet JM. Salinity and water stress have contrasting effects on the relationship between growth and cell viability during and after stress exposure in durum wheat callus. Plant Science. 2004(1);167:9‐18.

Available:https://doi.org/10.1016/j.plantsci.2004.02.014.

Rai MK, Kalia RK, Singh R, Gangola MP, Dhawan AK. Developing stress tolerant plants through in vitro selection—An overview of the recent progress. Environmental and Experimental Botany. 2010;71(1):89-98.

Available:https://doi.org/10.1016/j.envexpbot.2010.10.021

Ashraf M, Foolad MR. Crop breeding for salt tolerance in the era of molecular markers and marker- assisted selection. Plant Breeding. 2013;132:10‐20. Available:https://doi.org/10.1111/pbr.12000

Golkar P, Arzani A, Maibodi SAM. Evaluation of bread wheat (Triticum aestivum L.) cultivars for in vitro salt tolerance. Journal of Agriculture Science and Technology. 2007;20: 191‐200.

Errabii T, Gandonou CB, Essalmani H, Abrini J, Idaomar M, Skali-Senhaji N. Growth, proline and ion accumulation in sugarcane callus cultures under drought-induced osmotic stress and its subsequent relief. African Journal of Biotechnology. 2006;5(16):1488-1493. Available:https://www.researchgate.net/publication/27797557

Khorami A, Safarnejad A. In vitro selection of Foeniculum vulgare for salt tolerance. Notulae Science Biologicae.2011;3(2):90‐97. Available:https://doi.org/10.15835/nsb325806

Golkar P. Breeding improvements in safflower (Carthamus tinctorius L.): A review. Australian Journals of Crop Science. 2014;8(7):1079-1085.

Zebarjadi AR, Ghasempour HR, Soheilikhah Z. Effects of drought stress on biochemical and physiological parameters in callus cultures of Carthamus tinctorius varieties. Acta Agronomica Hungarica. 2010;58:395‐406.

Available:https://doi.org/10.1556/AAgr.58.2010.4.8

Baker CM, Dyer WE. Improvements in rooting regenerated safflower (Carthamus tinctorius L.) shoots. Plant Cell Reports. 1996;16:106‐110. Available:https://doi.org/10.1007/BF01275461

Basalma D, Uranbey S, Mirici S, Kolsarici. TDZ x IBA induced shoot regeneration from cotyledonary leaves and in vitro multiplication in safflower (Carthamus tinctorius L.). African Journal of Biotechnology. 2008;7(8): 960‐966.

Dhumale DR, Dudhare MS, Mohite NR, Shingote PR, Jadhav PV, Moharil MP. Refinement of in-vitro regeneration system in elite safflower (Carthamus tinctorius L.) genotypes. Journal of Plant Cell Tissue Research. 2015;15(1):4849-4854.

Kakaei M, Mansouri M, Abdollahi MR, Moradi F. Effect of NaCl and PEG induced osmotic stress on callus growth parameters of two Safflower (Carthamus tinctorius L.) cultivars. International Journal of Agriculture and Crop Sciences. 2013;6(3):127-132.

Errabii T, Gandonou CB, Essalmani H, Abrini J, Idaomar M, Senhaji NS. Effects of NaCl and mannitol induced stress on sugarcane (Saccharum sp.) callus cultures. Acta Physiologiae Plantarum. 2007;29:95‐102.

Available:https://doi.org/10.1007/s11738-006-0006-1

Woodward AJ, Bennett IJ. The effect of salt stress and abscisic acid on proline production, chlorophyll content and growth of in vitro propagated shoots of Eucalyptus camaldulensis. Plant cell, tissue and organ culture. 2005;82(2):189-200. Available:https://doi.org/10.1007/s11240-005-0515-4

Soheilikhah Z, Karimi N, Ghasmpour HR, Zebarjadi AR. Effects of saline and mannitol induced stress on some biochemical and physiological parameters of 'Carthamus tinctorius' L. varieties callus cultures. Australian Journal of Crop Science. 2013;7(12):1866-1874.

Rahman RA, Sara EG, Nader RA, Hossam MF, Wakil E, Khaled AS, Hassan H. Effect of sodium chloride on tropane alkaloids accumulation and proline content in Datura metel and D. stramonium callus cultures. International Journal of Advanced Biological and Biomedical Research. 2013;1(2):197-210.

Elyasi P, Farshadfar E, Aghaee M. Response of bread wheat genotypes to immature Embryo culture, callus induction and drought stress. Current Research Journal of Biological Sciences. 2012;4(4):372-380.

Pagariya MC, Devarumath RM, Kawar PG. Biochemical characterization and identification of differentially expressed candidate genes in salt stressed sugarcane. Plant science. 2012;184:1-13.

Available:https://doi.org/10.1016/j.plantsci.2011.12.002

Alagarasan G, Mani V, Rameshsing CN. In vitro screening for improving salinity tolerance in rice (Oryza sativa). The Bioscane (1 and 2). 2015:205- 208.

Sen J, Sharma AK. Micropropagation of withania somnifera from germinating seeds and shoot tips. Plant cell, tissue and organ culture.1991;26(2):71-73. Available:https://doi.org/10.1007/BF00036108

Belide S, Hac L, Singh SP, Green AG, Wood CC. Agrobacterium- mediated transformation of safflower and the efficient recovery of transgenic plants via grafting. Plant Methods. 2011;7:12. Available:https://doi.org/10.1186/1746-4811-7-12