Application of Auxins in Haploid Embryo Induction in Hexaploidy Wheat

Shubham *

Department of Genetics and Plant Breeding, Lovely Professional University, Phagwara, Punjab, India.

Sanjeet Singh Sandal

Department of Genetics and Plant Breeding, Lovely Professional University, Phagwara, Punjab, India.

Puneet Walia

Department of Genetics and Plant Breeding, Lovely Professional University, Phagwara, Punjab, India.

Vidushi Upadhyay

Department of Genetics and Plant Breeding, Lovely Professional University, Phagwara, Punjab, India.

*Author to whom correspondence should be addressed.


Doubled haploid (DH) plant production plays a crucial role in modern plant breeding programs, offering an efficient means to generate homozygous lines from heterozygous parents within a single generation. Different types of auxins have been utilized in wheat cross with maize DH production, with 2,4-D being the most widely used and effective hormone, followed by dicamba. Other auxins, including picloram, Indole-3-acetic acid (IAA), phenylacetic acid (PAA), silver nitrate, 1-naphthaleneacetic acid (NAA), kinetin, 6-benzyladenine (BA), and zearalenone, have also been tested for their potential role in haploid embryo induction. Various methods have been explored for the application of 2,4-D, such as spray, tiller injection, dipping, and spikelet culture methods. 2,4-D found to be most effective auxin treatment both alone and with combination with other phytohormones.

Keywords: Double haploid (DH), auxin, 2,4-dichlorophenoxyacetic acid (2,4-D), embryo recovery, haploid embryo induction, concentration, efficiency

How to Cite

Shubham, Sandal , S. S., Walia , P., & Upadhyay , V. (2023). Application of Auxins in Haploid Embryo Induction in Hexaploidy Wheat. International Journal of Environment and Climate Change, 13(9), 251–255.


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