Effects of Plant Growth-promoting Rhizobacteria (PGPR) and Arbuscular Mycorrhizal Fungi (AMF) on Morpho-Physiological Parameters of Strawberry cv. Chandler under Different Moisture Levels

Purnita Raturi *

Department of Horticulture, College of Agriculture, G.B. Pant University of Agriculture & Technology, Pantnagar – 263145, Uttarakhand, India.

Ratna Rai

Department of Horticulture, College of Agriculture, G.B. Pant University of Agriculture & Technology, Pantnagar – 263145, Uttarakhand, India.

A. K. Sharma

Department of Biological Sciences, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar – 263145, Uttarakhand, India.

A. K. Singh

Department of Horticulture, College of Agriculture, G.B. Pant University of Agriculture & Technology, Pantnagar – 263145, Uttarakhand, India.

D. C. Dimri

Department of Horticulture, College of Agriculture, G.B. Pant University of Agriculture & Technology, Pantnagar – 263145, Uttarakhand, India.

Gurdeep Bains

Department of Plant Physiology, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar – 263145, Uttarakhand, India.

*Author to whom correspondence should be addressed.


Abstract

Drought stress is one of the challenges that can affect the growth and quality of strawberries. The present study aims to determine the effect of PGPR and AMF on the growth of the strawberry cultivar Chandler under different moisture levels. This study was conducted under polyhouse at the Horticulture Research Center, GBPUA&T, Pantnagar, during 2021-2022 and 2022-23. The experiment was arranged in a factorial CRD design (FCRD) with three replications. The experiment included moisture levels (100% field capacity (FC), 75% FC and 50% FC) and bioinoculants: control (without PGPR or AMF), PGPR, AMF and combination of PGPR+AMF. The results showed that water stress significantly reduced all the growth and biochemical characteristics of leaves. Plants treated with PGPR and AMF either alone or in combination showed higher survival rate of plants as compared to control. The numbers of leaves, petiole length, chlorophyll a, chlorophyll b, total chlorophyll and carotenoids were found to increase in all the treatments where bioinoculants were incorporated in the potting media. The increase in chlorophyll content suggests improved photosynthetic efficiency which eventually enhanced stress tolerance of the plants. The use of PGPR and AMF provides a ray of hope for sustainable agricultural cultivation of strawberry particularly in regions affected by water scarcity.

Keywords: Plant growth-promoting rhizobacteria, arbuscular mycorrhizal fungi, drought stress, strawberry, bioinoculants


How to Cite

Raturi , Purnita, Ratna Rai, A. K. Sharma, A. K. Singh, D. C. Dimri, and Gurdeep Bains. 2023. “Effects of Plant Growth-Promoting Rhizobacteria (PGPR) and Arbuscular Mycorrhizal Fungi (AMF) on Morpho-Physiological Parameters of Strawberry Cv. Chandler under Different Moisture Levels”. International Journal of Environment and Climate Change 13 (9):2707-13. https://doi.org/10.9734/ijecc/2023/v13i92502.

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References

Nezhadahmadi A, Faruq G, Rashid K. Influence of drought stress on leaf traits of different strawberry (Fragaria ananassa l.) Varieties in natural environment. Commun. Soil Sci. 2015;46(10):1249–1262. DOI:10.1080/00103624.2015.1033536.

Chaves MM, Flexas J, Pinheiro C. Photosynthesis under drought and salt stress: Regulation mechanisms from whole plant to cell. Ann. Bot. 2009;103: 551–560. DOI:10.1093/aob/mcn125

Yenni Ibrahim MH, Nulit R, Sakimin SZ. Influence of drought stress on growth, biochemical changes and leaf gas exchange of strawberry (Fragaria x ananassa Dutch.) in Indonesia. AIMS Agric. Food. 2021;7(1):37-60.

Pandey RK, Maranville JW, Chetima MM. Deficit irrigation and nitrogen effects on maize in a Sahelian environment, II: Shoot growth. Agric. Water Manag. 2000;46: 15–27. DOI:10.1016/S0378-3774(00)00074-3.

Kaushal M, Wani SP. Plant-growth-promoting rhizobacteria: Drought stress alleviators to ameliorate crop production in drylands. Ann Microbiol. 2016;66:35– 42. DOI: 10.1007/S13213-015-1112-3.

Ahmad HM, Fiaz S, Hafeez S, Zahra S, Shah AN, Gul B, Aziz O, Fakhar A, Rafique M, Chen Y, Yang SH, Wang X. Plant growth-promoting rhizobacteria eliminate the effect of drought stress in plants: A Review. Front. Plant Sci. 2022; 13:875774. DOI:10.3389/fpls.2022.875774.

Chakraborti S, Bera K, Sadhukhan S, Dutta P. Bio-priming of seeds: Plant stress management and its underlying cellular, biochemical and molecular mechanisms. Plant Stress. 2022;3:100052. DOI: 10.1016/J.STRESS.2021.100052

Mickan BS, Abbott LK, Stefanova K, Solaiman ZM. Interactions between biochar and mycorrhizal fungi in a water-stressed agricultural soil. Mycorrhiza. 2016;1–10.

Auge RM. Water relations, drought and vesicular-arbuscular mycorrhizal symbiosis. Mycorrhiza. 2001;11:3–42.

Lichtenthaler HK, Wellburn AR. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem. Soc. Trans. 1983;603:591–592.

Azizi S, Tabari Kouchaksaraei M, Hadian J, Fallah Nosrat Abad AR, Modarres Sanavi SAM, Ammer C, Bader MKF. Dual inoculations of arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria boost drought resistance and essential oil yield of common myrtle. Forest Ecology and Management. 2021; 497:119478. DOI: 10.1016/j.foreco.2021.119478

Hsiao TC. Plant responses to water stress. Annu Rev Plant Physiol. 1973;24:519–70. DOI:10.1146/annurev.pp.24.060173.002511

Lisar SYS, Motafakkerazad R, Hossain MM, Rahman IMM. Water stress in plants: Causes, effects and responses. In: Rahman IMM, Hasegawa H, (Eds.). Water Stress, Croatia: InTech. 2012;1–14. DOI:10.5772/39363.

Asrar AA, Elhindi KM, Alleviation of drought stress of marigold (Tagetes erecta) plants by using arbuscular mycorrhizal fungi. Saudi J. Biol. Sci. 2011; 18:93–98.

Quintana LM, Moya M, Moya RS, Ariza AC, Rabert C, Pollmann S, Ramos P. Improvement in the physiological and biochemical performance of strawberries under drought stress through symbiosis with Antarctic fungal endophytes. Front. Microbiol. 2022;13: 939955. DOI: 10.3389/FMICB.2022.939955.

Begum N, Qin C, Ahanger MA, Raza S, Khan MI, Ashraf M, Ahmed N, Zhang L. Role of arbuscular mycorrhizal fungi in plant growth regulation: Implications in abiotic stress tolerance. Front. Plant Sci. 2019;10:466052. DOI:10.3389/fpls.2019.01068.

Kloepper JW. Biological control agents vary in specificity for host, pathogen control, ecological habitat and environmental conditions. Bio. Sci. 1996; 46:406–9.

Marius S, Octavita A, Eugen U, Vlad A. Study of a microbial inoculation on several biochemical indices in sunflower (Helianthus anuus L.). Genet. Mol. Biol. 2005;12:11–17.