In vitro Compatibility of Trichoderma and Bacillus biocontrol agents with Different Fungicides

K. Rajesh

Department of Plant Pathology, College of Agriculture, PJTSAU, Rajendranagar, India.

V. Ramya *

Department of Plant Pathology, College of Agriculture, PJTSAU, Rajendranagar, India.

S. Ameer Basha

Department of Plant Pathology, College of Agriculture, PJTSAU, Rajendranagar, India.

S. N. C. V. L. Pushpavalli

Institute of Biotechnology, College of Agriculture, PJTSAU, Rajendranagar, India.

K. Aravind

Department of Plant Pathology, College of Agriculture, PJTSAU, Rajendranagar, India.

*Author to whom correspondence should be addressed.


Biocontrol agents are beneficial for plant and soil health and are effective in controlling many plant diseases. These biocontrol agents in combination with fungicides at reduced doses can be more effective than using alone. Keeping this in view, an in vitro study was carried out to test the compatibility of commonly used fungicides viz., captan 50 WP, thiram 75% DS, tebuconazole 5.36% FS, carboxin 37.5% + thiram 37.5% DS, prochloraz 24.4% + tebuconazole 12.1% w/w EW and thiophanate methyl 450 g/l + pyraclostrobin 50 g/l with two fungal (Trichoderma asperellum and T.viride) and three bacterial biocontrol agents (Bacillus subtilis S4KB5, B. subtilis S8KB2 and B. subtilis B3). All the five fungicides were found to be compatible with all the biocontrol agents at 100ppm and 250ppm. All the fungicides except thiram, showed complete inhibition of T. asperellum at 1500ppm and 2000ppm and with T. viride, 100 per cent inhibition is shown by all the fungicides at 1500ppm and 2000ppm except carboxin + thiram. With biocontrol isolates, B. subtilis S4KB5, B. subtilis S8KB2 and B. subtilis B3, highest inhibition zone were recorded by prochloraz + tebuconazole (2000ppm), tebuconazole (2000ppm) and thiophanate methyl + pyraclostrobin (2000ppm). Present findings suggest that compatible fungicides can be used with biocontrol agents in an integrated disease management practices for the control of seed and soil borne pathogens.

Keywords: Biocontrol agents, sustainable agriculture, compatibility, fungicides, Trichoderma and Bacillus.

How to Cite

Rajesh , K., Ramya , V., Basha , S. A., Pushpavalli , S. N. C. V. L., & Aravind , K. (2023). In vitro Compatibility of Trichoderma and Bacillus biocontrol agents with Different Fungicides. International Journal of Environment and Climate Change, 13(9), 3340–3353.


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Baysal-Gurel F, Kabir N. Comparative performance of fungicides and biocontrol products in suppression of Rhizoctonia root rot in viburnum. Journal of Plant Pathology and Microbiology. 2018;9:451.

Panth M, Hassler SC, Baysal-Gurel F. Methods for management of soil borne diseases in crop production. Agriculture. 2020;10(1):16.

Pandey N, Singh M, Gupta S. Characterization of Bacillus isolates from Pea fields based on IAA production and growth promotion activities. Annuals of Plant Protection Sciences. 2015;23(2):313-318.

Zhao L, Xu Y, Lai XH, Shan C, Deng Z, Ji Y. Screening and characterization of endophytic Bacillus and Paenibacillus strains from medicinal plant Lonicera japonica for use as potential plant growth promoters. Brazilian Journal of Microbiology. 2015;46:977-989.

Kumar R, Singh SK, Yadav S, Kumar R, Choubey AK, Kumari A. Compatibility of Trichoderma viride with different fungicides and organic cake. Journal of Pharmacognosy and Phytochemistry. 2018;7(2):2398-2401.

Buck JW. Combination of fungicides with Phylloplane yeasts for improved control of Botrytis cinerea on geranium seedlings. Phytopathology. 2004;94:196-202.

Nene YL, Thapliyal PN. Fungicides in Plant Disease Control. Third edition Oxford and IBH publishing company, New Delhi. 1993;691.

Vincent JM. Distortion of fungal hyphae in the presence of certain inhibitors. Nature. 1947;159(4051):850.

Magaldi S, Mata-Essayag S, De Capriles CH, Pérez C, Colella MT, Olaizola C, Ontiveros Y. Well diffusion for antifungal susceptibility testing. International Journal of Infectious Diseases. 2004;8(1):39-45.

Valgas C, Souza SM, Smânia EF, Smania Jr. A. Screening methods to determine antibacterial activity of natural products. Brazilian Journal of Microbiology. 2007; 38:369-380.

Gomez KA, Gomez AA. Statistical Procedures for Agricultural Research. Second edition. John Wiley and Sons, New York. 1984;356.

Panse VS, Sukhatme PV. Statistical Methods for Agricultural Workers. Fourth edition. Indian Council of Agricultural Research. New Delhi. 1985;347.

McLean KL, Hunt J, Stewart A. Compatibility of the biocontrol agent Trichoderma harzianum C52 with selected fungicides. New Zealand Plant Protection. 2001;54:84-88.

Bagwan NB. Evaluation of Trichoderma compatibility with fungicides, pesticides, organic cakes and botanicals for integrated management of soil borne disease of soybean (Glycine max (L.) Merril). International Journal of Plant Protection. 2010;3(2):206-209.

Kay SJ, Stewart A. The effect of fungicides on fungal antagonists of onion white rot and selection of dicarboximide-resistant biotypes. Plant Pathology. 1994;43(5):863-871.

Nallathambi P, Umamaheswari C, Thakore BBL, More TA. Post-harvest management of ber (Ziziphus mauritiana Lamk) fruit rot (Alternaria alternata Fr. Keissler) using Trichoderma species, fungicides and their combinations. Crop Protection. 2009;28(6): 525-532.

Abd-El Moity TH, Papavizas GC, Shatla MN. Induction of new isolates of Trichoderma harzianum tolerant to fungicides and their experimental use for control of white rot of onion. Phytopathology. 1982;72(4):396-400.

Papavizas GC, Lewis JA, Moity THAE. Evaluation of new biotypes of Trichoderma harzianum for tolerance to benomyl and enhanced biocontrol capabilities. Phytopathology. 1982;72(1):126-132.

Mishra A, Sharma SD, Patel SI. Cross-tolerance of Trichoderma harzianum rifai to fungicides. Indian Journal of Agricultural Research. 2004;38(3):207-211.

Mohiddin FA, Khan MR. Tolerance of fungal and bacterial biocontrol agents to six pesticides commonly used in the control of soil borne plant pathogens. African Journal of Agricultural Research. 2013;8(43):5272-5275.

Singh M, Pandey N. Evaluation of antifungal activity of Bacillus spp. against Fusarium oxysporum and Rhizoctonia solani in Chick pea (Cicer arietinum L.). Annuals of Plant Protection Sciences. 2020;28(1):33-37.

Harsha MK, Daunde AT, Bhalerao PB, Sakhare SS. Compatibility studies of Bacillus spp. with commonly used agrochemicals. Pharma Innovation Journal. 2023.

Kishore GM, Jacob GS. Degradation of glyphosate by Pseudomonas sp. PG2982 via a sarcosine intermediate. Journal of Biological Chemistry. 1987;262(25):12164-12168.