Standard Operating Procedure for Micropropagation of Melia dubia Cav.: An Important Fastgrowing Tree

Sarita Bodalkar *

Department of Forestry, IGKV, Raipur -492001, Chhattisgarh, India.

R. K. Prajapati

Department of Forestry, IGKV, Raipur -492001, Chhattisgarh, India.

Ajit Mannade

Department of Plant Molecular Biology and Biotechnology, IGKV, Raipur-492001, Chhattisgarh, India.

S. P. Vishwakarma

Department of Forestry, IGKV, Raipur -492001, Chhattisgarh, India.

*Author to whom correspondence should be addressed.


Abstract

Due to increasing demand to meet out industrial requirement as a raw material , soft wood forest species are under tremendous pressure across the globe. The demand of fast growing Melia dubia is one of them. The usual approach of regeneration for this plant is through seed is unable to produce large scale plants. The present investigation aimed to develop the Standard Operating Procedure through Tissue culture method for mass multiplication of M.dubia using nodal segment . Results showed that the highest shoot initiation response (86.6%) was recorded in Murashige and Skoog medium supplemented with additives, NAA (0.1 mg l-1) and Kinetin (0.5mg l-1). Maximum response of shoot multiplication with highest shoot length of 5.5 cm was obtained in MS medium supplemented with combinations of Ascorbic acid (50 mg l-1) and Kinetin 1 mgl-1. For rhizogenesis, MS + 3.0 mgl-1 IBA (93.3 %) demonstrated superior in terms of the percentage of cultures with root induction, the average number of roots, and the average length of roots per explant. In conclusion present study ensures the successful mass multiplication of M. dubia, demonstrating the importance of tissue culture in the expansion of this economically significant multipurpose tree.

Keywords: M. dubia, shoot initiation, multiplication, response, explants


How to Cite

Bodalkar , S., Prajapati , R. K., Mannade , A., & Vishwakarma , S. P. (2023). Standard Operating Procedure for Micropropagation of Melia dubia Cav.: An Important Fastgrowing Tree. International Journal of Environment and Climate Change, 13(10), 25–30. https://doi.org/10.9734/ijecc/2023/v13i102728

Downloads

Download data is not yet available.

References

Mandang YI, Artistien S. Wood anatomy and fiber quality of utap-utap (Aromadendron elegans BI.) and seven other lesser known wood species. Bulletin Penelitian Hasil Hutan. 2003;21:111-127.

Bhimi Ram et al. An efficient protocol for micropropagation and genetic stability analysis of Melia dubia Cav. - An important multipurpose tree. International Journal of Current Microbiology and Applied Sciences. 2014;3(7):533-544.

Suprapti S, Djarwanto Hudiansyah. The resistance of five wood species against several wood destroying fungi. Jurnal Penelitian Hasil Hutan. 2004;22:239-246.

Parthiban KT, Bharathi AK, Seenivasan R, Kamala K, Rao MG. Integrating Melia dubia in agroforestry farms as an alternate pulpwood species. Asia Pac Agro News. 2009;34:3-4.

Chinnaraj S, Malimuthu C, Subrahmanyam SV. Development of micro-propagation and mini cutting protocol for fast growing Melia, Dalbergia and Eucalyptus clones for pulpwood and bio-energy plantations. In: Grattapaglia D (eds) From genomes to integration and delivery. Proc IUFRO Tree Biotechnology Conference, Bahia, Brazil. 2011;332-334.

Pettit GR. Anti-neoplastic agents: 489, Isolation and structure of meliastatins, 1–5 and related euphane triterpenes from tree Melia dubia. Journal of Natural Products. 2002;65:1886-1891

Nagalakshmi MAH, Thangadurai D, Pullaiah T. In vitro antimicrobial efficacy of leaf essential oils of Chukrasia tabularis Adr. Juss. and Melia dubia Cav. (Meliaceae). Phytotherapy Research. 2003;17(4):414- 416.

Vijayan P, Raghu C, Ashok G, Dhanaraj SA, Suresh B. Antiviral activity of medicinal plants of Nilgiris. Indian Journal of Medical Research. 2004;120:24-29

Gerige SJ, Ramjaneyulu. Antimicrobial activity of Melia dubia leaf volatile oil and camphene compound against skin pathogens. International Journal of Plant Sciences. 2007;2(2):166-168.

Susheela T, Balaravi P, Theophilus J, Reddy TN, Reddy PUM. Evaluation of hypoglycaemic and antidiabetic effect of Melia dubia CAV fruits in mice. Current Science. 2008;94(9):1191-1195.

Sukumaram S, Raj ADS. Medicinal plants of sacred groves in Kanyakumari district Southern Western Ghats. Indian Journal of Traditional Knowledge. 2010;9(2): 294-299.

Sharma R, Arya V. A Review on Fruits Having Anti-Diabetic Potential. Journal of Chemical and Pharmaceutical Research. 2011;3:204-212.

Mascarenhas AF, Hazara S, Potdar V, Kulkarani DK, Gupta PK. Rapid clonal multiplication of mature forest trees through tissue culture: In: Proceedings of the 5th International Congress on plant tissue and cell culture (Ed) A. Fujiwara Tokyo, Japan. 1982;719-720.

Kavitha RK, Smitha H, Souza LD. Micropropagation of Butea monosperma (Lam) Taub. Biotechnology. 1996;19:291-293.

Mallika Vanangamudi KT. Parthiban C, Surendran Annamalai R. Micropropagation of Azadirachta indica A. Juss. from nodal segments of three year old trees. Journal of Non Timber Forest Products. 1997;4(1/2):34-37.

Majumdar K, Sinha S, Sinha RK. In vitro regeneration and multiplication of shoots in Albizia procera Benth. Adv. Pl. Sci. 1998;11(2):7-12.

Parthiban KT, Malliga Vanangamudi C, Surendran, Balaji S. Callogenesis and organogenesis in Eucalyptus tereticornis. Indian J. For. 1999;22(2):101-105.

Devi YS, Mukherjee BB, Gupta S. Rapid cloning of elite teak (Tectona grandis L.) by In vitro multiple shoots proliferation. Indian J. Exp. Bio. 1994;32:668-671.

Kaur K, Verma B, Kant U. Plants obtained from the Khair tree (Acacia catechu Willd.) using mature nodal segments. Plant Cell Rep. 1998;17:427-429.

Husain MK, Anis M, Shahzad A. In vitro propagation of a multipurpose leguminous tree (Pterocarpous marsupium Roxb.) using nodal explants. Acta Physiol. Plant. 2008;30:353-359.

Husain MK, Anis M. Rapid In vitro multiplication of Melia azedarach L. (a multipurpose woody tree). Acta Physiol. Plant. 2009;31:765-772.

Sen A. et al. Pivotal role of plant growth regulators in clonal propogation of Melia azedarach. International Journal of Pharmaceutical Sciences Review and Research. 2010;5(2):43-49.

Sekar I. Seed storage, seed source variations, molecular characterization and In vitro propagation in Simarouba glauca DC. Ph. D. Thesis, Tamil Nadu Agricultural University, Coimbatore; 2003.

Kamala K. Screening of thornless bamboos for pulpwood quality and development of In vitro propagation techniques. M. Sc. Thesis, Tamil Nadu Agricultural University, Coimbatore; 2009.

Nesamani K. Studies on seed sources, micro propagation and molecular characterization of sex specificity in simarouba gluaca DC. M.Sc. Thesis, Tamil Nadu Agricultural University, Coimbatore; 2005.

Saravana Kumar VG. et al. Development of proliferation and acclimatization of Melia dubia-Australian teak variety through micropropagation. International Journal of Innovative Research in Science, Engineering and Technology. 2017;6(1): 1226-1236.

Shah Syed Naseer et al. An efficient protocol for in vitro organogenesis and antioxidant studies in Melia dubia Cav. African Journal of Biotechnology. 2016; 15(19):768-775.

Su WW, Hwang H, Kim SY, Sagawa Y. Induction of somatic embryogenesis in Azadirachta indica. Plant cell, Tissue and Organ Culture. 1997;50:91-95.