Enhancement of Vigour Status through Hydro and Leaf Extract Priming and Humid Invigoration in Ribbed Gourd (COH 1) and Bitter Gourd (CO 1)

Poovarasu K. *

Department of Seed Science and Technology, AC & RI, TNAU, Coimbatore - 641003, India.

R. Jegathambal

Krishi Vigyan Kendra, Sandhiyur, Salem – 636203, India.

R. Umarani

Directorate of Seed Centre, TNAU, Coimbatore - 641003, India.

P. S. Kavitha

Nammazhvar Organic Farming Research Centre, TNAU, Coimbatore - 641003, India.

M. Ravi

Krishi Vigyan Kendra, Sandhiyur, Salem – 636203, India.

*Author to whom correspondence should be addressed.


The present study focused on the evaluation of priming with water and various botanical leaf extract and followed by humid invigoration  as a means to improve seed germination and seedling vigour of ribbed gourd (COH 1)  and bitter gourd (CO 1). The seeds of these gourds were  primed with water  and various leaf extracts at 2% concentration viz., Pongam (Pongamia pinnata L.), Neem (Azadira chtaindica), Moringa (Moringa oleifera), Curry leaf (Murraya koenigii), Notchi (Vitex negundo), Coconut (Cocos nucifera), Hena (Lawsonia inermis) and Guava (Psidium guajava). Unprimed seeds taken as a control. Hydro and leaf extract priming with humid invigoration process significantly influence the germination and seedling vigour of both crops viz., ribbed gourd (COH 1) and bitter gourd (CO 1). The study revealed that highest seed and seedling quality characters like germination percentage, speed of germination, seedling length, dry matter production, vigour index I and vigour index II were recorded in hydro primed with humid invigoration. Among the leaf extracts moringa shows better seedling growth of above parameters. Minimum value of this parameter was recorded in henna leaf extract priming with humid invigoration in both ribbed gourd (COH 1) and bitter gourd gourd (CO 1).

Keywords: Botanical leaf extrct, seed priming, humid invigoration, bitter gourd, ribbed gourd

How to Cite

K. , P., Jegathambal, R., Umarani, R., Kavitha, P. S., & Ravi, M. (2023). Enhancement of Vigour Status through Hydro and Leaf Extract Priming and Humid Invigoration in Ribbed Gourd (COH 1) and Bitter Gourd (CO 1). International Journal of Environment and Climate Change, 13(10), 1333–1343. https://doi.org/10.9734/ijecc/2023/v13i102785


Download data is not yet available.


Dhillon NP, Laenoi S, Srimat S, Pruangwitayakun S, Mallappa A, Kapur A, et al. Sustainable cucurbit breeding and production in Asia using public–private partnerships by the world vegetable center. Agronomy. 2020;10(8):1171.

Nagarani G, Abirami A, Siddhuraju P. Food prospects and nutraceutical attributes of Momordica species: A potential tropical bioresources–A review. Food Science and Human Wellness. 2014;3(3-4):117-126.

Nadkarni K. Dr. KM Nadkarni’s Indian materia medica: with ayurvedic, unani-tibbi, siddha, allopathic, homeopathic, naturopathic & home remedies, appendices & indexes. 1. Pop. Prakashan. 1996;1:751-752.

Harris D, Joshi A, Khan P, Gothkar P, Sodhi P. On-farm seed priming in semi-arid agriculture: development and evaluation in maize, rice and chickpea in India using participatory methods. Experimental Agriculture. 1999;35(1):15-29.

Pill W, Finch‐Savage WE. Effects of combining priming and plant growth regulator treatments on the synchronisation of carrot seed germination. Annals of Applied Biology. 1988;113(2): 383-389.

Taylor A, Harman G. Concepts and technologies of selected seed treatments. Annual review of phytopathology. 1990; 28(1):321-339.

Afzal I, Basra S, Ahmad N. Hormonal priming induces salt tolerance in wheat through enhanced antioxidant defence system. Cereal Res. Commun. 2011;39: 334-342.

Bakht J, Shafi M, Jamal Y, Sher H. Response of maize (Zea mays L.) to seed priming with NaCl and salinity stress. Spanish Journal of Agricultural Research. 2011;9(1):252-261.

Chung IM, Miller DA. Effect of alfalfa plant and soil extracts on germination and growth of alfalfa. Agronomy Journal. 1995;87(4):762-767.

Ahmed DM, Nimer AM. Effects of Acacia senegal (L., Willd.) on sandy soils: A case study of El damokeya forest, Northern Kordofan State. University of khartoum Journal of Agricultural Sciences. 2002; 10(1):106-118.

Farooq M, Aziz T, Basra S, Cheema M, Rehman H. Chilling tolerance in hybrid maize induced by seed priming with salicylic acid. Journal of Agronomy and Crop Science. 2008;194(2):161-168.

Goutam M, Purohit R. Antimicrobial activity of the essential oil of the leaves of Murraya koenigii (Linn) Spreng (Indian Curry leaf). Indian J. Pharm. 1974;36(1): 11-12.

Chowdhury JU, Bhuiyan MNI, Yusuf M. Chemical composition of the leaf essential oils of Murraya koenigii (L.) Spreng and Murraya paniculata (L.) Jack. ||| Bangladesh Journal of Pharmacology. 2008;3(2):59-63.

Tamilmani U. Studies on effect of various seed management practices on quality seed production in greengram (Vigna radiata L.) cv. ADT; 2012.

Prakash M, Narayanan GS, Kumar, BS Kamaraj A. Effect of seed hardening and pelleting on seed quality and physiology of rice in aerobic condition. Agricultural Science Digest-A Research Journal. 2013;33(3):172-177.

Powell AA, Yule LJ, Jing HC, Groot SP, Bino RJ, Pritchard HW. The influence of aerated hydration seed treatment on seed longevity as assessed by the viability equations. Journal of Experimental Botany. 2000;51:2031-2043.

Nath S, Coolbear P, Hampton JG. Hydration-dehydration treatments to protect or repair stored Karamu wheat seeds. Crop Science.1991;31:822-826.

Mohammadi G, Koohi Y, Ghobadi M, Najaphy A. Effects of seed priming, planting density and row spacing on seedling emergence and some phenological indices of corn (Zea mays L.). Philippine Agricultural Scientist. 2014; 97(3):300-306.

Beata MK, Wyszyński Z, Pačuta V, Rašovský M, Różańska A. The effect of seed priming on field emergence and root yield of sugar beet. Plant, Soil and Environment. 2018;64(5):227-232.

Langeroodi A, Noora R. Seed priming improves the germination and field performance of soybean under drought stress. Journal of Animal and Plant Sciences. 2017;27(5):1611-1620.

Lekić S, Draganić I, Milivojević M, Todorović G. Germination and seedling growth response on sunflower seeds to priming and temperature stress. Helia. 2015;38(63):241-252.

Alias N, Billa L, Muhammad A, Singh A. Priming and temperature effects on germination and early seedling growth of some Brassica spp. III All Africa Horticultural Congress. 2016;1225.

Farooq M, Barsa SM, Wahid A. Priming of field-sown rice seed enhances germination, seedling establishment, allometry and yield. Plant Growth Regulation. 2006;49:285-294.

Forti C, Shankar A, Singh A, Balestrazzi A, Prasad V, Macovei A. Hydropriming and biopriming improve Medicago truncatula seed germination and upregulate DNA repair and antioxidant genes. Genes. 2020;11(3):242.

Jisha K, Puthur JT. Seed hydropriming enhances osmotic stress tolerance potential in Vigna radiata. Agricultural Research. 2018;7:145-151.

Jayesh V, Meeta J. Influence of halopriming and hydropriming on seed germination and growth characteristics of Zea mays L. cv. GSF-2 under salt stress. Research Journal of Chemistry and Environment. 2015;19:10.

Jisha KC, JT Puthur. Seed halopriming outdo hydropriming in enhancing seedling vigor and osmotic stress tolerance potential of rice varieties. Journal of Crop Science and Biotechnology. 2014;17:209-219.

Casenave E, M. Toselli. Germination of melon seeds under water and heat stress: Hydropriming and the hydrotime model. Seed Science and Technology. 2010; 38(2):409-420.

SAĞLAM S, Sibel D, Gamze K, Gürbüz A. Hydropriming increases germination of lentil (Lens culinaris Medik.) under water stress. Notulae Scientia Biologicae. 2010; 2(2):103-106.

Pandita V, Anand A, Nagarajan S. Enhancement of seed germination in hot pepper following presowing treatments. Seed Science and Technology. 2007; 35(2):282-290.

Devika OS, Singh S, Sarkar D, Barnwal P, Suman J, Rakshit A. Seed priming: A potential supplement in integrated resource management under fragile intensive ecosystems. Frontiers in Sustainable Food Systems. 2021;5: 654001.

Saleem MS, M Sajid, Z Ahmed, S Ahmed, N Ahmed, S Islam. Effect of seed soaking on seed germination and growth of bitter gourd cultivars. J Agric Vet Sci. 2014; 6(6):7-11.

Vijayalakshmi K, Sundaralingam K. Enhancement of Vigour Status through Micronutrient Priming and Humid Invigouration in Sesame. Madras Agricultural Journal. 2018;105:40- 43.

Thornton JM, Collins AR, Powell AA. The effect of aerated hydration on DNA synthesis in embryos of Brassica oleracea L. Seed Science Research. 1993;3:195-199.

Khan MM, Qasim M, Iqbal MJ, Naeem AF, Abbas M. Effect of seed humidification on germinability, vigour and leakage in cockscomb (Celosia argentia var. cristata L.). International Journal of Agriculture and Biology. 2003;5:499-503.

Simons DH, Sfakiotakis E, Dilley DR. Enhancement of in vitro pollen germiantion of lily with increased pre-inoculation humidity. HortScience; 1972.

Loupassaki M, Vasilakakis M, Androulakis I. Effect of pre-incubation humidity and temperature treatment on the in vitro germination ofavocado pollen grains. Euphytica. 1997;94:247-251.

Henny RJ. Pollen germination in aglaonema flowers of different ages.HortScience (USA); 1988.

Luza JG, Polito VS, Weinbaum SA. Staminate bloom date and temperature responses of pollen germination and tube growth in two walnut (Juglans) species. American Journal of Botany.

Sinha M, Das DK, Bhattacharjee S, Majumdar S, Dey S. Leaf extract of Moringa oleifera prevents ionizing radiation-induced oxidative stress in mice. Journal of Medicinal Food. 2011;14(10): 1167-1172.

Foidl N, Makkar H, Becker K. What development potential for Moringa products. A conference onon the potential of Moringa oleifera for Agricultural and industrial uses. October 20th–November 2nd; 2001.

Christiansen M, Foy C. Fate and function of calcium in tissue. Communications in Soil Science and Plant Analysis. 1979;10(1-2):427-442.

Nandhakumar M. Utilization of leaves and leaf extracts of tropical trees for eco-friendly Vrkshayurvedic farming in maize (Zea mays L.). MSc (Agric) thesis, Tamil Nadu Agricultural University, Coimbatore, TN, India; 2010.

Shehzad M, Ayub M, Ahmad A, Yaseen M. Influence of priming techniques on emergence and seedling growth of forage sorghum (Sorghum bicolor L.). J Anim Plant Sci. 2012;22(1):154-158.

Suguna A. Scientific study on Vrkshayurvedic farming in barnyard millet (Echinochloa frumentaceae L.). M. Sc.(Agriculture) Thesis; 2012.

Kaveriammal S, Geethambigai S, Subramani A. Phytotoxicity effects of Lawsonia inermis L. on the seed germination and growth performance of selected pulses. International Journal of Research in Botany. 2013;3(1):23- 26.

Namkeleja HS, Tarimo MT, Ndakidemi PA. Allelopathic effects of Argemone mexicana to growth of native plant species. American Journal of Plant Sciences; 2014.

Chapla TE, Campos JB. Allelopathic evidence in exotic guava (Psidium guajava L.). Brazilian Archives of Biology and Technology. 2010;53:1359-1362.

Khan AR, Khan MJ, Anwar M, Gull P, Fatima M. Allelopathic effect of different concentrations of leaf extract of Lawsonia inermis L., seed germination of Steria italica, Pennisetum americanum and Lectuca sativa. Int. J. Pharma. and Phytochem. Res. 2014;6:766- 773.