CaO Nanoparticles Seed Invigoration on Germination and Seedling Growth of Rice var. TRY 3

S. Gopinath *

Department of Seed Science and Technology, Tamilnadu Agricultural University, Coimbatore, India.

K. Raja

Department of Plant Breeding and Genetics, ADAC&RI, Trichy, India.

V. Manonmani

Department of Seed and Technology, TNAU, Coimbatore, India.

P. Jeyaprakash

Department of Seed Science and Technology, Tamilnadu Agricultural University, Coimbatore, India.

T. Ramesh

Department of Agronomy, ADAC&RI, Trichy, India.

C. Vanniarajan

ADAC&RI, Trichy, India.

S. Kavitha

Seed Centre, TNAU, India.

*Author to whom correspondence should be addressed.


Rice (Oryza sativa L.), a vital cereal and staple food crop in South East Asia. It faces significant challenges in cultivation worldwide, due to soil salinity. Seed enhancement techniques like priming have shown resilience against salt stress. In this study, the potential of nano-priming with calcium oxide nanoparticles (CaO NPs) to enhance rice seed germination and growth was investigated. CaO NPs were synthesized from organic waste (eggshells) using a sol-gel technique and characterized using Raman and UV-Vis spectroscopy. Paddy seeds were primed with varying concentrations of CaO NPs, and their germination and seedling growth were assessed. The results showed that nano-priming with 40 ppm of CaO NPs significantly improved germination, root and shoot length, dry matter production, and seedling vigour as compared to the control. However, higher concentrations of CaO NPs had a detrimental effect on germination. The study suggests that CaO NP seed priming has the potential to enhance rice germination and seedling development.

Keywords: Rice, calcium oxide nanoparticle, nanopriming, seedling vigour

How to Cite

Gopinath , S., K. Raja, V. Manonmani, P. Jeyaprakash, T. Ramesh, C. Vanniarajan, and S. Kavitha. 2023. “CaO Nanoparticles Seed Invigoration on Germination and Seedling Growth of Rice Var. TRY 3”. International Journal of Environment and Climate Change 13 (10):2160-67.


Download data is not yet available.


Lutts S, Kinet J, Bouharmont J. Changes in plant response to NaCl during development of rice (Oryza sativa L.) varieties differing in salinity resistance. Journal of Experimental Botany. 1995;46(12):1843-1852.

Khan MA, Abdullah Z. Salinity–sodicity induced changes in reproductive physiology of rice (Oryza sativa) under dense soil conditions. Environmental and Experimental Botany. 2003;49(2):145-157.

Ghassemi F, Jakeman AJ, Nix HA. Salinisation of land and water resources: 429 human causes, extent, management and case studies. CABI Publishing, Wallingford. 1995;526.

Gupta S, Schillaci M, Walker R, Smith PM, Watt M, Roessner U. Alleviation of salinity stress in plants by endophytic plant-fungal symbiosis: Current knowledge, perspectives and future directions. Plant and Soil. 2021;461:219-244.

Rabbani G, Rahman A, Mainuddin K. Salinity-induced loss and damage to farming households in coastal Bangladesh. International Journal of Global Warming. 2013;5(4): 400-415.

Solis CA, Yong MT, Vinarao R, Jena K, Holford P, Shabala L, Chen ZH. Back to the wild: On a quest for donors toward salinity tolerant rice. Frontiers in Plant Science. 2020;11: 323.

Jerlin R, Menaka C, Raja K, Moorthy KR, Tamilkumar P. Standardization of sieve size for grading of olitorius jute seeds. Asian Journal of Agricultural Research, 2010;4(1):15-19.

Krishnasamy V, Srimathi P. Seed Management of rainfed agriculture. Land and use planning and watershed management in Rainfed Agriculture (ed.) Balusamy, M., CR Chinnamuthu and A. Velayutham. Centre of Advanced studies. Department of Agronomy, Tamilnadu Agricultural University, Coimbatore. 2001;140.

Raja K, Sowmya R, Sudhagar R, Sathya MP, Govindaraju K, Subramanian KS. Biogenic ZnO and Cu nanoparticles to improve seed germination quality in blackgram(Vigna mungo). Materials Letters. 2019;235:164-167.

Mukiri C, Raja K, Senthilkumar M, Subramanian KS, Govindaraju K, Pradeep D, Ranjan S. Immobilization of beneficial microbe Methylobacterium aminovorans in electrospun nanofibre as potential seed coatings for improving germination and growth of groundnut Arachis hypogaea. Plant Growth Regulation. 2021;1-9.

Dhage S, Biradar DP. Nanofertilizers: Perspective to enhance growth, yield and NUE of crops. Indian Journal Pure and Applied Biosciences. 2020;8(6):339-49.

Alam A, Ullah H, Cha-um S, Tisarum R, Datta A. Effect of seed priming with potassium nitrate on growth, fruit yield, quality and water productivity of cantaloupe under water-deficit stress. Scientia Horticulturae. 2021;288:110354.

Zhang W, et al. Silicon promotes growth and root yield of glycyrrhiza uralensis under salt and drought stresses through enhancing osmotic adjustment and regulating antioxidant metabolism. Crop Protection. 2018;107:1-11.

Habte L, Shiferaw N, Mulatu D, Thenepalli T, Chilakala R, Ahn JW. Synthesis of nano-calcium oxide from waste eggshell by sol-gel method. Sustainability. 2019;11(11): 3196.

Jaiswal KK, Dutta S, Pohrmen CB, Verma R, Kumar A, Ramaswamy AP. Bio-waste chicken eggshell-derived calcium oxide for photocatalytic application in methylene blue dye degradation under natural sunlight irradiation. Inorganic and Nano-Metal Chemistry. 2020;51(7):995-1004.

Abdul‐Baki AA, Anderson JD. Vigor determination in soybean seed by multiple criteria 1. Crop science. 1973;13(6):630-633.

Marquis G, Ramasamy B, Banwarilal S, Munusamy AP. Evaluation of antibacterial activity of plant mediated CaO nanoparticles using Cissus quadrangularis extract. Journal of Photochemistry and Photobiology B: Biology. 2016;155:28-33.

Fu C, Khan MN, Yan J, Hong X, Zhao F, Chen L, Wu H. Mechanisms of nanomaterials for improving plant salt tolerance. Crop and Environment; 2023.

Khodakovskaya M, Dervishi E, Mahmood M, Xu Y, Li Z, Watanabe F, Biris AS.Carbon nanotubes are able to penetrate plant seed coat and dramatically affect seed germination and plant growth. ACS nano. 2009;3(10):3221-3227.

Sami F, Yusuf M, Faizan M, Faraz A, Hayat S. Role of sugars under abiotic stress. Plant Physiology and Biochemistry. 2016;109:54-61.

Mazhar MW, Ishtiaq M, Maqbool M, Atiq Hussain S, Casini R, Abd-ElGawad AM, Elansary HO. Seed Nano-Priming with Calcium Oxide Maintains the Redox State by Boosting the Antioxidant Defense System in Water-Stressed Carom (Trachyspermum ammi L.) Plants to Confer Drought Tolerance. Nanomaterials. 2023;13(9):1453.

Bewley JD, et al. Seeds: Germination, structure, and composition. Seeds: physiology of development and germination. 1994;1-33.

Aroca R, R Porcel, Ruiz-Lozano JM. Regulation of root water uptake under abiotic stress conditions. Journal of Experimental Botany. 2012;63(1):43-57.

Mulaudzi T, Hendricks K, Mabiya T, Muthevhuli M, Ajayi RF, Mayedwa N, Iwuoha E. Calcium improves germination and growth of Sorghum bicolor seedlings under salt stress. Plants. 2020;9(6):730.

Ayesha Farooq, Sumera Javad, Khajista Jabeen, Anis Ali Shah, Asma Ahmad, Adnan Noor Shah, Mohammed Nasser Alyemeni, Walid F.A Mosa, Asad Abbas. Effect of calcium oxide, zinc oxide nanoparticles and their combined treatments on growth and yield attributes of Solanum lycopersicum L.Journal of King Saud University – Science. 2023;35(5).

Alberto García-González, Lluvia de Abril Alexandra Soriano-Melgar, María Luisa Cid-López,Gladis Yakeline Cortez-Mazatán, Esmeralda Mendoza-Mendoza, Luis Alonso ValdezAguilar, René Darío Peralta-Rodríguez,Effects of calcium oxide nanoparticles on vase life of gerbera cut flowers,Scientia Horticulturae. 2023;291.

Javadi A, Khomari S, Sofalian O. Seed vigor and boron and calcium nutrition influence oilseed rape germinability and seedling growth under salt stress. Journal of Plant Nutrition. 2016;39(12):1688-1696.