Exploration of Exopolysaccharide Producing Bacterial Cultures from Various Drought Affected Region
N. Niranjani *
Department of Agricultural Microbiology, Agricultural College and Research Institute, TNAU, Madurai-625104, India.
K. Kumutha
Department of Agricultural Microbiology, Agricultural College and Research Institute, TNAU, Madurai-625104, India.
R. Subhashini
Department of Agricultural Microbiology, Agricultural College and Research Institute, TNAU, Madurai-625104, India.
P. Kannan
Department of Soils and Environment, Agricultural College and Research Institute, TNAU, Madurai-625104, India.
M. L. Mini
Department of Biochemistry, Agricultural College and Research Institute, TNAU, Madurai-625104, India.
*Author to whom correspondence should be addressed.
Abstract
Exopolysaccharides (EPS) are extracellular macromolecules secreted by microorganisms play a vital role in improving soil aggregation as well as enhancing the water holding capacity of the soils. This study focuses onto the isolation and characterization of bacterial strains capable of producing Exopolysaccharides (EPS) from drought-affected soil regions across different districts of Tamil Nadu. The core objective revolves around the molecular identification of elite cultures and pivotal genes, responsible for EPS production namely dex (dextransucrase) by using the primer pair WConDex fw and WConDex rev at an amplification size of 1037 bp size. A total of 72 bacterial cultures were isolated from 67 soil samples that are capable of secreting EPS using specific medium.
Keywords: Exopolysaccharides, phenol sulfuric acid assay, dextran gene
How to Cite
Downloads
References
Bensalim S, Nowak J, Asiedu SK. A plant growth promoting rhizobacterium and temperature effects on performance of 18 clones of potato. American Journal of Potato Research. 1998;75:145-52.
Naseem H, Bano A. Role of plant growth-promoting rhizobacteria and their exopolysaccharide in drought tolerance of maize. Journal of Plant Interactions. 2014;9(1):689-701.
Timmusk S, Abd El-Daim IA, Copolovici L, Tanilas T, Kännaste A, Behers L, Nevo E, Seisenbaeva G, Stenström E, Niinemets Ü. Drought-tolerance of wheat improved by rhizosphere bacteria from harsh environments: Enhanced biomass production and reduced emissions of stress volatiles. Plos One. 2014;9(5): e96086.
DOI: 10.1371/journal.pone.0096086. PMID: 24811199; PMCID: PMC4014485.
Ventorino V, Nicolaus B, Di Donato P, Pagliano G, Poli A, Robertiello A, Iavarone V, Pepe O. Bioprospecting of exopolysaccharide-producing bacteria from different natural ecosystems for biopolymer synthesis from vinasse. Chemical and Biological Technologies in Agriculture. 2019;6:1-9.
Andhare P, Goswami D, Delattre, Cedric Delattre, Guillaume Pierre, Phillipe Michaud, Hilor Pathak. Edifying the strategy for the finest extraction of succinoglycan from Rhizobium radiobacter strain CAS. Appl Biol Chem. 2017;60:339–348.
Available:https://doi.org/10.1007/s13765-017-0286-8
Deka P, Goswami G, Das P, Gautom T, Chowdhury N, Boro RC, Barooah M. Bacterial exopolysaccharide promotes acid tolerance in Bacillus amyloliquefaciens and improves soil aggregation. Molecular Biology Reports. 2019;46:1079-91.
Jahan S, Chowdhury SF, Mitu SA, Shahriar M, Bhuiyan MA, Banats J. Genomic DNA extraction methods: A comparative case study with gram–negative organisms. Biotechnol. 2015;6(11):61-8.
Malang SK, Maina NH, Schwab C, Tenkanen M, Lacroix C. Characterization of exopolysaccharide and ropy capsular polysaccharide formation by Weissella. Food Microbiology. 2015;46:418-27.
Green MR, Sambrook J. Polymerase chain reaction. Cold Spring Harbor Protocols. 2019;(6):pdb-top095109.
Noble RT, Weisberg SB. A review of technologies for rapid detection of bacteria in recreational waters. Journal of Water and Health. 2005;3(4):381-92.
Kaci Y, Heyraud A, Barakat M, Heulin T. Isolation and identification of an EPS-producing Rhizobium strain from arid soil (Algeria): characterization of its EPS and the effect of inoculation on wheat rhizosphere soil structure. Research in Microbiology. 2005;156(4):522-31.
Hunter RC, Beveridge TJ. High-resolution visualization of Pseudomonas aeruginosa PAO1 biofilms by freeze-substitution transmission electron microscopy. Journal of bacteriology. 2005;187(22): 7619-30.
Jurášková D, Ribeiro SC, Silva CC. Exopolysaccharides produced by lactic acid bacteria: From biosynthesis to health-promoting properties. Foods. 2022;11 (2):156.
Chenu C. Clay—or sand—polysaccharide associations as models for the interface between micro-organisms and soil: Water related properties and microstructure. Soil Structure/Soil Biota Interrelationships. 1993:143-56.
Angelin J, Kavitha M. Exopolysaccharides from probiotic bacteria and their health potential. International Journal of Biological Macromolecules. 2020;162: 853-65.