Harnessing the Antifungal Potential of Seed Bacteriome against Major Seed and Soil-borne Pathogens in Blackgram

Chandana K. C. *

Department of Seed Science and Technology, Tamil Nadu Agriculture University, Coimbatore, India.

S. Lakshmi

Open Distance and Learning, TNAU, Coimbatore, India.

T. Anand

Seed Centre, TNAU, Coimbatore, India.

S. Kavitha

Seed Centre, TNAU, Coimbatore, India.

C. Sharmila Rahale

Centre for Agricultural Nanotechnology, TNAU, Coimbatore, India.

*Author to whom correspondence should be addressed.


Abstract

Blackgram is an important pulse crop in India. One of the major constraints on blackgram production is the attack of pathogens, which leads to yield loss. Nowadays, with the understanding of harmful effects of chemical fungicides, biocontrol methods are gaining more importance. One such method is biopriming with endophytes to control pathogens and enhance yield. In the present study, the endophytes of blackgram seeds was explored and tested for its biocontrol potential against major seed- and soil-borne pathogens and growth promotion in blackgram. A total of 14 bacterial endophytic isolates were obtained and screened against Macropomina phaseolina and Fusarium oxysporum. Among them, BSE5 isolate was proven to be more efficient in inhibiting the mycelial growth of M. phaseolina and F. oxysporum, with per cent inhibition of 22.5 and 50.6, respectively, following BSE9. Both the isolates showed improved plant growth parameters like germination percentage, root length, shoot length, dry matter production and vigour index. Molecular characterisation of BSE5 and BSE9 confirmed that the isolates are Pseudomonas aeruginosa and Bacillus xiamenensis.

Keywords: Blackgram, endophytes, Fusarium oxysporum, Macropomina phaseolina, seed priming


How to Cite

K. C. , C., Lakshmi , S., Anand , T., Kavitha , S., & Rahale , C. S. (2023). Harnessing the Antifungal Potential of Seed Bacteriome against Major Seed and Soil-borne Pathogens in Blackgram . International Journal of Environment and Climate Change, 13(10), 2699–2708. https://doi.org/10.9734/ijecc/2023/v13i102934

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References

Ghosh PK, Hazra KK, Nath CP, Das A, Acharya CL. Scope, constraints and challenges of intensifying rice (Oryza sativa) fallows through pulses. Indian J Agron. 2016;61(4):122-128.

Kumar GS, Ramesh T, Subrahmaniyan K, Ravi V. Effect of sprinkler irrigation levels on the performance of blackgram (Vigna mungo) varieties. Legume Research-An International Journal. 2018;41(2):299-302.

Sunil Kulkarni M, Shobharani, Raja. Integrated management of dry root rot of blackgram caused by rhizoctonia bataticola. Int. J. Curr. Microbiol. App. Sci. 2019; 8(08):853-858.

Wrather A, Shannon G, Balardin R, Carregal L, Escobar R, Gupta GK, Tenuta A. Effect of diseases on soybean yield in the top eight producing countries in 2006. Plant Health Progress. 2010;11(1): 29.

Vishalakshi B, Umakanth B, Shanbhag AP, Ghatak A, Sathyanarayanan N, Madhav MS, Yadla H. RAPD assisted selection of black gram (Vigna mungo L. Hepper) towards the development of multiple disease resistant germplasm. 3 Biotech. 2017;7:1-6.

Pal G, Kumar K, Verma A, Verma SK. Seed inhabiting bacterial endophytes of maize promote seedling establishment and provide protection against fungal disease. Microbiological Research. 2022;255: 126926.

Al-Nadabi HH, Al-Buraiki NS, Al-Nabhani AA, Maharachchikumbura SN, Velazhahan R, Al-Sadi AM. In vitro antifungal activity of endophytic bacteria isolated from date palm (Phoenix doctylifera L.) against fungal pathogens causing leaf spot of date palm. Egyptian Journal of Biological Pest Control. 2021;31:1-8.

Amadi JE, Adeleke EE, Olahan G, Garuba T, Omoniyi AM. Effect of plant extracts on sporulation and spore germination of stored melon seed fungi; 2014.

Veraja P, Rai P. Effect of polymer coating, chemicals and biocontrol agent on storability of black gram (Vigna mungo L.). International Journal of Plant & Soil Science. 2015;8(6):1-8.

Ladumor SA, Singh P, Shekhada MR, Panara KN. Field effect of seed bio priming on leaf blight (Macrophomina phaseolina) of Black gram; 2022.

Mukherjee A, Singh BK, Verma JP. Harnessing chickpea (Cicer arietinum L.) seed endophytes for enhancing plant growth attributes and bio-controlling against Fusarium sp. Microbiological Research. 2020;237:126469.

Morton DJ, Stroube WH. Antagonistic and stimulating effects of soil microorganism of Sclerotium. Phytopath. 1955;45:417- 420.

Vincent JM. The esters of 4‐hydroxybenzoic acid and related compounds. Part I. Methods for the study of their fungistatic properties. Journal of the Society of Chemical Industry. 1947; 66(5):149-155.

Abdul‐Baki AA, Anderson JD. Vigor determination in soybean seed by multiple criteria 1. Crop Science. 1973;13(6):630-633.

Reddy YTN, Khan MM. Effect of osmopriming on germination, seedling growth and vigour of khirni (Mimusops hexandra) seed. Seed Research. 2001; 29(1):24-27.

Lorck H. Production of hydrocyanic acid by bacteria. Physiologia Plantarum. 1948;1(2): 142-146.

Bric JM, Bostock RM, Silverstone SE. Rapid in situ assay for indole-acetic acid production by bacteria immobilized on a nitrocellulose membrane. Applied and environmental Microbiology. 1991;57(2): 535-538.

Santhanam R, Luu VT, Weinhold A, Goldberg J, Oh Y, Baldwin IT. Native root-associated bacteria rescue a plant from a sudden-wilt disease that emerged during continuous cropping. Proceedings of the National Academy of Sciences. 2015; 112(36):E5013-E5020.

Cha JY, Han S, Hong HJ, Cho H, Kim D, Kwon Y, Kwak YS. Microbial and biochemical basis of a Fusarium wilt-suppressive soil. The ISME Journal. 2016; 10(1):119-129.

Silva Santos SD, Silva AAD, Polonio JC, Polli AD, Orlandelli RC, Oliveira JADS, et al. Influence of plant growth-promoting endophytes Colletotrichum siamense and Diaporthe masirevici on tomato plants (Lycopersicon esculentum Mill.). Mycology. 2022;13(4):257-270.

Gupta S, Pandey S, Sharma S. Decoding the plant growth promotion and antagonistic potential of bacterial endophytes from Ocimum sanctum Linn. against root rot pathogen Fusarium oxysporum in Pisum sativum. Frontiers in Plant Science. 2022;13:813686.

Gupta CP, Sharma A, Dubey RC, Maheshwari DK. Pseudomonas aeruginosa (GRC~ 1) as a strong antagonist of Macrophomina phaseolina and Fusarium oxysporum. CYTOBIOS-CAMBRIDGE-. 1999;183-189.

Levy E, Gough FJ, Berlin KD, Guiana PW, Smith JT. Inhibition of Septoria tritici and other phytopathogenic fungi and bacteria by Pseudomonas fluorescens and its antibiotics. Plant Pathology. 1992;41(3): 335-341.

Gupta V, Kumar GN, Buch A. Colonization by multi-potential Pseudomonas aeruginosa P4 stimulates peanut (Arachis hypogaea L.) growth, defence physiology and root system functioning to benefit the root-rhizobacterial interface. Journal of Plant Physiology. 2020;248:153144.

Islam MA, Nain Z, Alam MK, Banu NA, Islam MR. In vitro study of biocontrol potential of rhizospheric Pseudomonas aeruginosa against Fusarium oxysporum f. sp. cucumerinum. Egyptian Journal of Biological Pest control. 2018;28:1-11.

Sulochana MB, Jayachandra SY, Kumar SA, Parameshwar AB, Reddy KM, Dayanand A. Siderophore as a potential plant growth-promoting agent produced by Pseudomonas aeruginosa JAS-25. Applied Biochemistry and Biotechnology. 2014; 174:297-308.

Xia Y, Farooq MA, Javed MT, Kamran MA, Mukhtar T, Ali J, Chaudhary HJ. Multi-stress tolerant PGPR Bacillus xiamenensis PM14 activating sugarcane (Saccharum officinarum L.) red rot disease resistance. Plant Physiology and Biochemistry. 2020; 151:640-649.

Haidar R, Roudet J, Bonnard O, Dufour MC, Corio-Costet MF, Fert M, Fermaud M. Screening and modes of action of antagonistic bacteria to control the fungal pathogen Phaeomoniella chlamydospora involved in grapevine trunk diseases. Microbiological Research. 2016;192:172-184.

Thoa NTK, Mai DTH, Hiu BL, Duong CA, Chau NNB, Nghiep NM, Quoc NB. Roles of β-Indole Acetic Acid (IAA) Producing Endophytic Bacteria on the Recovery of Plant Growth and Survival Ability of Sugarcane Infected White Leaf Disease (SWLD). Current Microbiology. 2022; 79(12):389.

Hwang HH, Chien PR, Huang FC, Yeh PH, Hung SHW, Deng WL, Huang CC. A plant endophytic bacterium Priestia megaterium Strain BP-R2 Isolated from the Halophyte Bolboschoenus planiculmis enhances plant growth under salt and drought stresses. Microorganisms. 2022;10(10): 2047.