Enhancing Crop Resilience through CRISPR/Cas9-Mediated Development of Disease-Resistant Cultivars

Ajeet Kumar Gupta

Department of Genetics and Plant Breeding, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya-224229, Uttar Pradesh, India.

Govind Mishra

Department of Genetics and Plant Breeding, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya-224229, Uttar Pradesh, India.

Harikant Yadav

Department of Genetics and Plant Breeding, G. B. Pant University of Agriculture and Technology, Pantnagar (263145), Uttrakhand, India.

Rishabh Gupta

Department of Genetics and Plant Breeding, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya-224229, Uttar Pradesh, India.

Abhay Singh

Department of Genetics and Plant Breeding, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya-224229, Uttar Pradesh, India.

Jay Singh *

Department of Seed Science and Technology, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur, Uttar Pradesh, India.

Piyusha Singh

Department of Genetics and Plant Breeding, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya-224229, Uttar Pradesh, India.

*Author to whom correspondence should be addressed.


A major issue in agriculture is the protection of crops against diseases and pests. Plant breeding has been primarily responsible for the growth of disease-resistant cultivars. The use of gene editing techniques in plant breeding is essential for obtaining desired features. Clustered Regular Interspaced Palindromic Repeats (CRISPER)/Cas9 (CRISPR-related protein) is a new advancement in gene editing technology. It can be utilised in plant defence mechanisms against pathogen attack by recognising the bacterial immune system and destroying invasive pathogen genes. Advances in plant breeding through CRISPR/Cas9 integration have helped develop cultivars including hereditary resistance to bacterial and viral diseases. Future crop generations can acquire CRISPR/Cas9-mediated transgene resistance if the Cas9/sgRNA transgene has been isolated in the F1 generation. Cas9/sgRNA transgene separation makes CRISPR/Cas9 safe for use in plant breeding. Although CRISPR/Cas9 has proven to be a wonderful tool to revolutionize plant breeding and develop various disease resistant varieties, its effect on many plant physiological processes remains to be thoroughly investigated.

Keywords: CRISPR/Cas9, gene editing, genome, plant breeding, resistance breeding

How to Cite

Gupta , Ajeet Kumar, Govind Mishra, Harikant Yadav, Rishabh Gupta, Abhay Singh, Jay Singh, and Piyusha Singh. 2023. “Enhancing Crop Resilience through CRISPR/Cas9-Mediated Development of Disease-Resistant Cultivars”. International Journal of Environment and Climate Change 13 (10):2773-83. https://doi.org/10.9734/ijecc/2023/v13i102942.


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