A Comprehensive Review of CRISPR/Cas9 Based Strategies in Horticultural Crop Modification
Gaurav Sharma
Department of Genetics and Plant Breeding, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, U.P- 250110, India.
Shivani Ahalawat *
Department of Genetics and Plant Breeding, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, U.P- 250110, India.
Asif Islam
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, 141004, India.
S. Vignesh
Department of Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, India.
Soumya Unnikrishnan
Department of Plantation, Spices, Medicinal and Aromatic Crops, Kerala Agricultural University, Trivandrum, Kerala, India.
Pradeep Kumar
Department of Agricultural Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, U.P- 250110, India.
Atar Singh
College of Agriculture, Department of Genetics and Plant Breeding, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, U.P- 250110, India.
Megha Raghavan
Department Fruit Science, College of Horticulture and Forestry, Central Agricultural University, Pasighat, Arunachal Pradesh 791102, India.
Prerna Singh
College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, U.P- 250110, India.
*Author to whom correspondence should be addressed.
Abstract
The introduction of CRISPR/Cas9 gene editing technology represents a groundbreaking advancement in the realm of horticulture. It provides a precise and effective means for making targeted genetic alterations in a wide range of plant species. This abstract delves into the diverse applications of CRISPR/Cas9 within the domain of horticultural crops, with a particular emphasis on its crucial role in tackling issues related to climate change. This review paper outlines the diverse applications of CRISPR/Cas9 in horticulture, including trait improvement for enhanced stress tolerance, disease resistance, and yield optimization. It highlights specific examples of successful CRISPR-edited horticultural crops and their contributions to climate adaptation. Furthermore, it discusses the potential of CRISPR/Cas9 in accelerating the development of new crop varieties tailored to thrive in changing climatic conditions. Additionally, the ethical and regulatory considerations surrounding the use of CRISPR/Cas9 in horticulture are addressed, as they play a crucial role in determining the broader adoption of this technology. Balancing the benefits of climate-resilient crop development with potential environmental and societal implications remains a critical aspect of its application. In conclusion, the transformative potential of CRISPR/Cas9 in horticultural crop improvement and its role in addressing climate-related challenges. By harnessing the power of genetic editing, horticulturalists can create more productive crops and better equipped to withstand the uncertainties of a changing climate. However, this technology's responsible and ethical use is imperative to ensure its long-term sustainability and benefit to society.
Keywords: Breeding, mango, physiological problems, disease and insect resistance