Protein and Total Free Amino Acid Content Evaluation of Improved and Local Varieties/Germplasm of Spine Gourd Momordica dioica Roxb. (Family - Cucurbitaceae)
Pankaj Kumar
Department of Biochemistry, Acharya Narendra Deva University of Agriculture and Technology, Ayodhya, India.
Jay Singh *
Department of Seed Science and Technology Chandra Shekhar Azad University of Agriculture and Technology Kanpur, India.
R. N. Kewat
Department of Biochemistry, Acharya Narendra Deva University of Agriculture and Technology, Ayodhya, India.
Ajay Pratap Singh
Department of Seed Science and Technology Chandra Shekhar Azad University of Agriculture and Technology Kanpur, India.
Prem Kumar
Department of Genetic and Plant Breeding, Chandra Shekhar Azad University of Agriculture and Technology Kanpur, India.
Yogendra Pratap Singh
Department of Seed Science and Technology Chandra Shekhar Azad University of Agriculture and Technology Kanpur, India.
*Author to whom correspondence should be addressed.
Abstract
Spine gourd (Momordica dioica Roxb., 2n = 2X= 28) is a nutritionally rich, perennial and dioecious cucurbit with good assortment of adaptability. Varieties of spine gourd improved germplasm/varieties collected from farmers' field and were sown in Completely Randomized Design (CRD) with Three replications at Acharya Narendra Deva University of Agriculture and Technology Ayodhya, Uttar Pradesh, India during monsoon season 2020. Biochemical characteristics of spine gourd analysis revealed that maximum Protein content (3.09mg/100g) was found in variety Kheri-3,1. Total free amino acid content (6.90mg/100g) was found in variety NDMD-2. Minimum protein content was found in the variety Arka Bharat (2.94mg/100g) and Total Free Amino acid content was reported in the variety Ambika-K-13,6 (6.27mg/100g). Based on observation, variety Kheri-3,1 and NDMD-2 were found superior in both Protein and Total Free Amino acid parameters followed by germplasm Arka Bharat and Ambika-13,6 respectively.
Keywords: Protein, total free amino acid, saponin M. dioica (SMD), dioecious