Semi Automated Movement Based Screening of Aqueous Extracts of Cinnamon Leaf, Almond, Beetroot Carrot, Ashwagandha and Tulsi on Caenorhabditis Elegans Model System for Health Beneficial Impact
Kisan B. *
Department of Molecular and Agricultural Biotechnology, University of Agricultural Sciences, Raichur, Karnataka (584104), India.
Vinay
Department of Molecular and Agricultural Biotechnology, University of Agricultural Sciences, Raichur, Karnataka (584104), India.
Radhika
Department of Molecular and Agricultural Biotechnology, University of Agricultural Sciences, Raichur, Karnataka (584104), India.
Ayyangouda Patil
Department of Agricultural Entomology, University of Agricultural Sciences, Raichur, Karnataka (584104), India.
Sharanbasappa Yerri
Department of Molecular and Agricultural Biotechnology, University of Agricultural Sciences, Raichur, Karnataka (584104), India.
Savitha A. S.
Department of Molecular and Agricultural Biotechnology, University of Agricultural Sciences, Raichur, Karnataka (584104), India.
Udaykumar Nidoni
Department of Molecular and Agricultural Biotechnology, University of Agricultural Sciences, Raichur, Karnataka (584104), India.
Shruthi Nagaral K.
Department of Molecular and Agricultural Biotechnology, University of Agricultural Sciences, Raichur, Karnataka (584104), India.
*Author to whom correspondence should be addressed.
Abstract
The research was conducted at University of Agricultural Sciences Raichur during 2021-22. In this research Caenorhabditis elegans is utilized by the researchers to know the effects of various extracts, drugs, nano particles, xenobiotics, magnetic effects on behaviour, neuronal effects, feeding pathways, apoptosis, ageing related pathways. Experiments were performed to evaluate the traditional herbs (Cinnamon, Almond, Beetroot Carrot, Ashwagandha and Tulsi) consumption for their health beneficial effects by monitoring the movement as an end point. Movement of an organism is considered as the health and age influencing factor. As the movement is well maintained in organism means health is also well maintained. Hence consistent movement is the factor for the healthy life. In the present study the organism movement has been monitored from L4 onwards every day for 30 minute till 21st day of the survival by automated system. The worm movement during this period has been categorised based on activity in three categories from L4 to 4days the activity ranged from 28.0 units to 10 IR units, 5th to 15th day 27.82 to 14.67 and from 16th to 21st day ranged from 17.87 to 14.44 IR units in non exposed N2 wild type worms. There is a gradual increase in the activity has been recorded on the exposure to health beneficial extracts in a single dose of supplementation. Hence, the study concludes that the health supplements can impact on the move ability of worms and maintains consistent movement till 21 days. Further studies are required to establish comprehensive health beneficial effects of these extracts at organism level.
Keywords: Cinnamon, almond, beetroot, carrot, ashwagandha, Tulsi, C. elegans