Advancement in Tissue Culture Techniques for Fruit Crops

Shubham Jain

Department of Fruit Science, College of Horticulture and Forestry, ANDUAT, Kumarganj, Ayodhya, India.

Rajat Sharma

GBPUAT, Pantnagar, Uttarakhand Now, Serving as District Horticulture Officer or Equivalent, Govt. of Haryana, India.

Nivedita Nidhi

Jharkhand Government, M.Sc. Horticulture, SHUATS, Prayagraj, U.P., India.

Swosti Debapriya Behera

School of Agriculture, GIET University, Gunupur, Odisha, India.

Hari Baksh

Department of Horticulture, Tilak Dhari PG College, Jaunpur – 222002, Uttar Pradesh, India.

Rohit Sharma

Dr. YSP UH&F Nauni, Solan (HP)- 173230, India.

Satyam Mishra

Department of Horticulture (Fruit science) SHUATS, Prayagraj, Naini, Uttar Pradesh.

Vikas Kumar

RCP College of Allied Sciences, Kishanpur, Hardwar, India.

Anushi *

Department of Fruit Science, CSAUAT, Kanpur, Uttar Pradesh (208002), India.

*Author to whom correspondence should be addressed.


Tissue culture is a highly promising approach that enables the efficient propagation of many plants from tiny fragments of the parent plant within a relatively brief timeframe and confined area. Tissue culture, a contemporary approach, is primarily employed for the efficient and extensive replication of many commercially significant plant species, such as the date palm. Utilizing the tissue culture technique presents a potential approach for generating a substantial quantity of genetically homogeneous palm plants that resemble other plants and yield typical fruit within four years from initial planting. Furthermore, this technique allows to produce date palm plants devoid of diseases, exhibiting an exceptionally high survival rate of nearly 100% when compared to the traditional vegetative propagation of shoots, owing to the robustness of their root system. The process of surface sterilization holds significant importance in the production of explants for in vitro studies, as it effectively addresses the issue of bacterial and fungal contamination originating from field sources, which might vary considerably across different fruit plant species. The efficacy of tissue culture techniques for date palm acclimatization in vitro is contingent upon the observation of leaf count prior to transplantation in the greenhouse. Hence, the primary objective of this study was to investigate the determinants that govern the tissue culture of fruit trees. India is known for being the native land of various fruit crops that are both significant and minor in terms of their importance. These crops include Indian gooseberry (Emblica officinalis Gaertn.), Karonda (Carissa carandas L.), Bael (Aegle marmelos Corr.), Jamun (Syzygium cuminii L.), and jackfruit (Artocarpus heterophyllus L.), etc. These fruits possess considerable nutritional, medicinal, and therapeutic value, making them highly valuable in commercial sectors such as medicine, food, and cosmetics. The limited availability of suitable planting materials imposes constraints on the commercial production process for these crops. Using plant tissue culture techniques holds promise in substantially augmenting the number of novel cultivars or genotypes inside fruit crops. The primary aim of this review study is to consolidate and synthesize the extant body of knowledge about the tissue culture techniques employed in cultivating various fruit crops.

Keywords: Tissue culture, In vitro, Somaclonal variation, Embryo rescue, Molecular marker

How to Cite

Jain , Shubham, Rajat Sharma, Nivedita Nidhi, Swosti Debapriya Behera, Hari Baksh, Rohit Sharma, Satyam Mishra, Vikas Kumar, and Anushi. 2023. “Advancement in Tissue Culture Techniques for Fruit Crops”. International Journal of Environment and Climate Change 13 (11):4396-4407.


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