Fungicides and Bioagents: Evaluation of Pathogen Eradication under Laboratory Conditions
Kanade Saurabh
Plant Pathology Section, College of Agriculture, Dhule 424 004, Mahatma Phule Krishi Vidyapeeth Rahuri, 413722, Maharashtra, India.
Wagh Sandip
College of Agriculture, Nandurbar, 425412, MPKV Rahuri, Maharashtra, India.
Khaire Pravin *
Department of Plant Pathology and Microbiology, Mahatma Phule Krishi Vidyapeeth, Rahuri, 413722, Maharashtra, India.
Gawali Rushikesh
Entomology Section, College of Agriculture, Dhule 424004, MPKV Rahuri, Maharashtra, India.
Thorat Vaishnav
Botany Section, (Genetics and Plant Breeding), College of Agriculture Dhule, 424004, MPKV Rahuri, Maharashtra, India.
*Author to whom correspondence should be addressed.
Abstract
In an in-vitro study evaluating eight different fungicides against C. musae, carbendazim, carbendazim 12% + mancozeb 63%, and azoxystrobin 11% + tebuconazole 18.3% exhibited 100% inhibition of fungal growth at concentrations of 0.1%, 0.25%, and 0.1%, respectively. Propiconazole (0.05%) was also the most effective fungicide, showing 83.03% mycelial inhibition, followed by carboxin 37.5% + thiram 37.5% (73.51%) and chlorothalonil (76.84%) at concentrations of 0.25% and 0.2%, respectively. Mancozeb exhibited the minimum mycelial inhibition (66.44%) at a concentration of 0.25%, while copper oxychloride showed the least inhibition (48.96%) at 0.25% concentration. In addition, five biocontrol agents were screened for linear growth inhibition of C. musae through the dual culture method. Among the Trichoderma isolates, T. harzianum demonstrated the highest growth inhibition (84.38%), followed by Pseudomonas fluorescens (80.16%).
Keywords: C. musae, bioagents, efficacy, inhibition, potential biocontrol agents