Fungicides and Bioagents: Evaluation of Pathogen Eradication under Laboratory Conditions

Kanade Saurabh

Plant Pathology Section, College of Agriculture, Dhule 424 004, Mahatma Phule Krishi Vidyapeeth Rahuri, 413722, Maharashtra, India.

Wagh Sandip

College of Agriculture, Nandurbar, 425412, MPKV Rahuri, Maharashtra, India.

Khaire Pravin *

Department of Plant Pathology and Microbiology, Mahatma Phule Krishi Vidyapeeth, Rahuri, 413722, Maharashtra, India.

Gawali Rushikesh

Entomology Section, College of Agriculture, Dhule 424004, MPKV Rahuri, Maharashtra, India.

Thorat Vaishnav

Botany Section, (Genetics and Plant Breeding), College of Agriculture Dhule, 424004, MPKV Rahuri, Maharashtra, India.

*Author to whom correspondence should be addressed.


Abstract

In an in-vitro study evaluating eight different fungicides against C. musae, carbendazim, carbendazim 12% + mancozeb 63%, and azoxystrobin 11% + tebuconazole 18.3% exhibited 100% inhibition of fungal growth at concentrations of 0.1%, 0.25%, and 0.1%, respectively. Propiconazole (0.05%) was also the most effective fungicide, showing 83.03% mycelial inhibition, followed by carboxin 37.5% + thiram 37.5% (73.51%) and chlorothalonil (76.84%) at concentrations of 0.25% and 0.2%, respectively. Mancozeb exhibited the minimum mycelial inhibition (66.44%) at a concentration of 0.25%, while copper oxychloride showed the least inhibition (48.96%) at 0.25% concentration. In addition, five biocontrol agents were screened for linear growth inhibition of C. musae through the dual culture method. Among the Trichoderma isolates, T. harzianum demonstrated the highest growth inhibition (84.38%), followed by Pseudomonas fluorescens (80.16%).

Keywords: C. musae, bioagents, efficacy, inhibition, potential biocontrol agents


How to Cite

Saurabh , Kanade, Wagh Sandip, Khaire Pravin, Gawali Rushikesh, and Thorat Vaishnav. 2023. “Fungicides and Bioagents: Evaluation of Pathogen Eradication under Laboratory Conditions”. International Journal of Environment and Climate Change 13 (12):559-65. https://doi.org/10.9734/ijecc/2023/v13i123713.

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References

Anonymous. FAOSTAT Agriculture data; 2022. Accessed on: July 2022.

National Horticultural Board, (NHB, 2022, 1st Advance Estimate), agriexchange.apeda.gov.in.

Gopalan C, Rama Sastri BV, Balasubramanian SC. Nutritive value of Indian foods, National Institute of Nutrition, ICMR, Hyderabad; 2004.

Rashad RA, Al-Najada AR, Saleh AM. Isolation and identification of some fruit spoilage fungi: Screening of plant cell wall degrading enzymes. Afri. J. Microbiol. Res. 2011;5(4):443-448.

Droby S, Zhu Improving quality and safety of fresh fruits and vegetables after harvest and the use of biological control and natural materials. Acta Hortic. 2006;709: 45-51.

Khaire PB, Hake LG. Some important post harvest diseases of tomato and their management. Popular Kheti. 2018;6(3):80-86.

Clay K. Fungal endophytes of grasses: A defensive mutualism between plants and fungi. Ecology. 1988; 69:10-16.

Photita W, Lumyong S, Lumyong P, McKenzie EHC, Hyde KD. Are some endophytes of Musa acuminata latent pathogens? Fungal Diversity 2004;16:131-140.

Amani M, Avagyan G, Sarpeleh A. Anthracnose disease of banana fruits in Iran. Bulletin of State University of Armenia; 2011.

Ershad D. Fungi of Iran. Plant Pests & Diseases Research Institute, Tehran. 1995;277.

Jones DR. Diseases of Banana, Abaca & Enset. CAB Intrnational. 2000;544.

Ploetz RC, Zentmyer GA, Nishijima WT, Rohrbach, KG, Ohr HD. Compendium of tropical fruit diseases. APS Press. The American phytopath. Society; 1994.

Sing RS. Diseases of Fruit Crops. Published by Scince Publisher, Inc., Enfield, NH, USA; 2000.

Stover RH. Banana, plantain and Abaca diseases. Commonw. Mycol, Instit., Kew; 1972.

Stover RH. Diseases of Banana and Abaca. Longman Scientific & Technical; 1972.

Wardlaw CW. Banana Diseases. John Wiley & Sons, New yourk; 1961.

Wardlaw CW. Banana Diseases Including plantains and abaca, 2nd edn. Longman, London; 1972.

Ranathunge NP, Rajapaksha RJ, Yogarajah K, Preethikumara M. Isolation, screening and in vitro evaluation of bacterial antagonist from spent mushroom substrate against Colletotrichum musae. Trop. Agric. Res. & Ext. 2014;17(2):115-120.

Dennis KL, Webster J. Antagonistic properties of species group of Trichoderma and hyphal interaction. British Mycol. Soc. 1971;57:363-396.

Vincent JM. Distoration of fungal hyphae in the presence of certaion inhibitors. Nature. 1947;159:159-850.

Kolase SV, Kamble TM, Musmade NA. Efficacy of different fungicides and botanicals against blossom blight of Mango caused by Colletotrichum gloeosporioides. Int. J. Plant Prot. 2014;7(2):444-447.

Rathva AA, Mehta BP, Chauhan R, Ganvit MR. In vitro evaluation of fungicides against Colletotrichum gloeosporioides Penz. and Sacc. causing anthracnose in pointed gourd. Int. J. Chemical Studies. 2017;5(6):1870-1872.

Somashekhara YM, Prasannakuma MK, Dev D. Cultural characterization of Colletotrichum gloeosporioides causing anthracnose of pomegranate. Indian Phytopathological Society; 2018.

Deshmukh PP, Raut JG. Antagonism by Trichoderma spp. on five plant pathogenic fungi. New Agriculturist. 1992;3:127-130.

Singh N. Biocontrol of red rot disease of sugarcane. Indian Phytopath. 1992;43:64.

Azad CS, Srivastav JN, Chand G. Evaluation of bio-agents for controlling anthracnose of banana caused by Colletotrichum musae in-vitro condition. The Bioscan. 2013;8(4):1221-1224.

Bhuvaneswari V, Rao MS. Evaluation of Trichoderma viride antagonistic to post harvest pathogens on mango. Indian Phytopathol. 2001;54(4):493-494.

Sangeetha G, Usharani S, Muthukumar A. Biocontrol with Trichoderma species for the management of postharvest crown rot of banana. Phytopathol. Mediterr. 2009; 48(2):214–225.

Shirshikar GS. Studies on fruit rots of mango (Mangifera indica L.) caused by Botryodiplodia theobromae and Colletotrichum gloeosporioides and their management. M. Sc. (Agri.) Thesis, B.S.K.K.V, Dapoli, Maharashtra; 2002.

Costa DM, De and Subasinghe SSNS. Antagonistic bacteria associated with the fruit skin of banana in controlling its post-harvest diseases. Trop. Sci. 1998;38(4): 206-212.

Pawar SV, Khaire PB, Mane SS. Management Strategies Used against Fungal Diseases of Capsicum. AgriCos e-Newsletter. 2020;1(5):22-26.

Patil PP. Studies on leaf blight of sapota caused by Colletotrichum gloeosporioides Penz. M.Sc. (Agri.) Thesis submitted to B.S.K.K.V., Dapoli, Maharashtra, India; 2009.

Lokhande R.D, Tiwari S, Patil RV. Eco-friendly management of anthracnose of chilli (Capsicum annuum L.) caused by Colletotrichum capsici (Syd.) Butler and Bisby. Int. J. Curr. Microbiol. App. Sci. 2019;8(2):1045-1052.

Asalkar UA, Hingole DG, Khaire PB, Mete VS. Effect of different solid media on the growth and sporulation of Colletotrichum gloeosporioides Penz. and Sacc. causing Fruit Rot of Aonla. Int.J. Curr. Microbiol. App. Sci. 2019;8(10):610-616. Available:https://doi.org/10.20546/ijcmas.2019.810.069

Rasha RA, Al-Najada AR, Saleh AM. Isolation and identification of some fruit spoilage fungi: Screening of plant cell wall degrading enzymes. Afri. J. Microbiol. Res. 2011;5(4):443-448.