Effect of Auxin and Cow Urine as Nutrient Source on Pineapple Propagation through Crown in Soilless Culture under Protected Condition

Kishan J. Prajapati

Department of Fruit Science, College of Horticulture, JAU, Junagadh, Gujarat, India.

Sharad K. Bhuva

Department of Fruit Science, College of Horticulture, JAU, Junagadh, Gujarat, India.

Mansi B. Shingala

Department of Floriculture and Landscape Architecture, College of Horticulture, JAU, Junagadh, Gujarat, India.

Pavan K. Patel *

Department of Horticulture, B. A. College of Agriculture, AAU, Anand, Gujarat, India.

*Author to whom correspondence should be addressed.


Abstract

The present study was aimed to investigate the effect of auxin and cow urine on rooting, survival and growth of pineapple through crown propagation with soilless culture under protected condition. The research was conducted in 2020 at the High-Tech Horticultural Park, College of Horticulture, Junagadh Agricultural University, Junagadh, employing a Completely Randomized Design (CRD) as the experimental design. Eight treatments were implemented, including Control (T1), IBA 400 ppm (T2), IBA 600 ppm (T3), IBA 800 ppm (T4), NAA 600 ppm (T5), NAA 800 ppm (T6), NAA 1000 ppm (T7) and Cow urine 15 % (T8). The results of this experiment revealed that IBA 600 ppm had a notable impact on various growth parameters. It exhibited the highest percentage of rooted crowns (94.44 %), length of root (5.58, 8.26 and 11.08 cm) at 15, 25 and 35 days, respectively, length of leaf (13.35, 16.27 and 19.67 cm) at 15, 25 and 35 days, respectively, no. of leaves (70.58, 80.28 and 91.06) at 15, 25 and 35 days, respectively. Furthermore, IBA 600 ppm significantly influenced the fresh root weight (16.46 g), dry root weight (7.77 g), fresh shoot weight (136.33 g), and dry shoot weight (57.67 g). Additionally, IBA 600 ppm demonstrated the highest survival rate (65.91%) and the lowest mortality rate (24.09%). While T6 gave maximum no. of roots (70.58, 80.28 and 91.06) at 15, 25 and 35 days, respectively. T8 gave minimum days required for root initiation (2.67 days). The use of IBA at 600 ppm for a brief dipping period, along with the utilization of NAA at 800 ppm for enhancing root formation, and Cow urine at 15% for accelerated rooting, can be recommended for promoting successful pineapple crown propagation.

Keywords: Pineapple, propagation, crown, PGR, cow urine and soilless


How to Cite

Prajapati, K. J., Bhuva , S. K., Shingala, M. B., & Patel , P. K. (2024). Effect of Auxin and Cow Urine as Nutrient Source on Pineapple Propagation through Crown in Soilless Culture under Protected Condition. International Journal of Environment and Climate Change, 14(2), 353–362. https://doi.org/10.9734/ijecc/2024/v14i23950

Downloads

Download data is not yet available.

References

Santos PBD, Barbosa FDS, Vieira CF, Carvalho ACPP. Number of explants, culture medium and photoperiod in micropropagation of ornamental pineapple. Agricultural Science Magazine. 2015;46:749-754.

Raheem G, Alharbi K, Mughrbil K, Jan A, Glolam A. Comparison between growing plants in hydroponic system and soil-based system. In Proceedings of the 4th World Congress on Mechanical, Chemical, and Material Engineering, Madrid, Spain: ICMIE. 2018;1-7.

Tamhane RV, Motiramani VP, Bali YP, Donahue RL. Manures, compost, green manure, saw dust and sewage. In soils, their chemistry and fertility in tropical Asia. Ed. IInd Prentice Hall of India, Pvt. Ltd., New Delhi. 1965;278-285.

Agrawal SN. Gozaran (Gomutra ka mahatwa). In Lok kalyan setu, 16 Aug to 15 Sept. 2002;3-6.

Adelaja BA. Rapid on-farm propagation techniques for plantain and banana. In Proceedings of 1st International Symposium on Banana and Plantains for Africa. Eds; 2000.

Panse V, Sukhatme P. Statistical methods for agricultural workers. ICAR, New Delhi. 1985;361.

Jackson ML. Soil chemical analysis. Prentice-Hall of India Pvt. Ltd., New Delhi; 1973.

Pawar JT, Khandekar RG, Mali PC, Mane AV. Effect of different plant growth promoters on sprouting and survival in bush pepper (Piper nigrum L.). Journal of Pharmacognosy and Phytochemistry. 2020;9(6):1886-1888.

Nanda KK. Physiology of adventitious root formation. Indian Journal Plant Physiology. 1975;18: 80-89.

Darwati I, Suryadi R, Syakir M. peningkatan keberhasilan sambungan top-working jambu mete (Annacardium Occidentale L.) dengan aplikasi asam indol butirat/improve grafting success of top working on cashew (Annacardium occidentale L.) by application of Indole Butyric Acid (IBA). Industrial Crops Research Journal. 2017;23(2):83-89.

Singh AK, Singh R, Ashutosh MK, Singh YP, Jahuri S. Effect of plant growth regulators on survival, rooting and growth characters in long pepper (Piper longum L.). Progressive Horticulture. 2003;35:208-211.

Satpal M, Rawat SS, Singh KK. Effect of various concentrations of IBA, type of cuttings and planting time on the rooting of cuttings of lemon (Citrus limon burm.) cv. Pant lemon-1 under valley conditions of Garhwal Himalaya, International Journal of Current Research. 2014;6(12):10974-10976.

Tien LH, Chac LD, Oanh LTL, Ly PT, Sau HT, Hung N, Thanh VQ, Doudkin RV, Thinh BB. Effect of auxins (IAA, IBA and NAA) on clonal propagation of Solanum procumbens stem cuttings. Plant Cell Biotechnology and Molecular Biology. 2020;21(55-56):113-120.

Bora N, Lal RL, Singh AK. Effect of IBA and planting containers on shoot and root characters and survival of Litchi air layers. Indian Journal of Horticulture. 2006;63(2): 155-158.

Kumar V, Singh MK, Kumar M, Prakash S, Kumar A, Rao S, Malik S. Effect of different doses of IBA and rooting media on rooting of stem cutting of lemon (Citrus limon Burm) cv. Pant Lemon-1. Journal of Plant Development Sciences. 2015;7(7): 587-591.

Shah K, Amin NU, Ahmad I, Ara G. Impact assessment of leaf pigments in selected landscape plants exposed to roadside dust. Environmental Science and Pollution Research. 2018;25(23):23055-23073.

Bahadar W, Khan J, Ghani A, Azam MK, Zafar SN, Aslam MZ, Rehman AU, Nazir F, Babar AMM, Ullah I. Effect of Indole Butyric Acid (IBA) on growth and success rate of hydrangea and fuchsia cuttings. Plant Science Journal. 2022;11(1):01-06.

Rout S, Khare N, Beura S. Vegetative propagation of Ashoka (Saraca asoca Roxb. De Wilde.) by stem cuttings. The Pharma Innovation Journal. 2018;7(1):486-488.

Carvalho Pires M, Peixoto JR, Yamanishi OK. Rooting of passion fruit species with indole-3-butyric acid under intermittent misting conditions. International Symposium on Tropical Horticulture. 2010; 894:177-183.

Haissing BE. Influence of auxin and auxin synergists on adventitious root primordium, initiation and development. New Zealand Journal of Forestry. 1974;4:311- 312.

Kaur S, Cheema SS, Chhabra BR, Talwar KK. Chemical induction of physiological changes during adventitious root formation and bud break in grapevine cuttings. Plant Growth Regulation 2002;37:63-68.

Murthy G, Umesha K, Gingade Smitha, Krishnamanohar R. Effect of growth regulators and bio-inoculants on rooting and growth of vanilla stem cuttings. Indian Journal of Horticulture. 2010;67:90-93.

Bhagya HP, Sreeramu BS. Effect of growth regulators on vegetative propagation of Vitex negundo L., Asian Journal of Horticulture. 2013;8(1):209-212.

Choudhri BK, Chakrawar VR. Effect of seed treatment using some chemicals on the shoot and root length of Rangpur lime (Citrus limonia Osbeck). Journal of Maharashtra Agriculture University. 1981; 7(1):66-68.

Alexander TR. Carbohydrates of bean plants after treatment with indole-3-acetic acid. Plant physiology. 1938;13(4):845.

Ali Mohamed, Saif Magdi. The impact of some treatments on improving grape cuttings rooting. Hortscience Journal of Suez Canal University. 2020;1:227-231

Ghosh D, Bandyopadhyay A, Sen S. Effect of NAA and IBA on adventitious root formation in stem cuttings of Pomegranate (Punica granatum L.) under intermittent mist. Indian Agriculturist. 1988;32(4):329-342.