Facultative Bacterial Diversity Associated with Silverleaf Whitefly, Bemisia tabaci (Gennadius) on Tomato Crop

Kishor Pujar *

Department of Agricultural Entomology, University of Agricultural Sciences, Bangalore-560065, India.

Jemla Naik D.

Department of Agricultural Entomology, University of Agricultural Sciences, Bangalore-560065, India.

Shivanna B.

Department of Agricultural Entomology, University of Agricultural Sciences, Bangalore-560065, India.

*Author to whom correspondence should be addressed.


Abstract

The adults and nymphs of Bemisia tabaci were collected on tomato crop from different locations of Karnataka during 2021-2023. Bacterial colonies were isolated from adults and nymphs of B. tabaci using spread-plate technique and identified through 16srRNA sequencing. Nymphs showed high (55%) abundance of bacteria than adults (45%). 63.64% of the bacterial population in the nymphs belong to the phylum Bacillota followed by pseudomonadota (36.36%). In adults, Bacillota found dominant (100%). The class Bacilli was dominant in both nymphs and adults (63.64 and 100% respectively). In the nymphal stage, Bacillales was dominant order (54.55%). Similarly, in adults also Bacillales was found dominant (77.79%). Bacillaceae was abundant in nymphs (45.45%)and in adults same family accounted for 66.67%. The genus Bacillus was dominant in both nymphs (45.45%) and adults (55.55%). The species, B. licheniformis, B. pumilus, B. safensis and Staphylococcus saprophyticus were found common between adults and nymphs. The bacterial diversity varies with the different stages of the B. tabaci on same host.

Keywords: 16srRNA, bacillus, Bemisia tabaci and facultative bacteria


How to Cite

Pujar, Kishor, Jemla Naik D., and Shivanna B. 2024. “Facultative Bacterial Diversity Associated With Silverleaf Whitefly, Bemisia Tabaci (Gennadius) on Tomato Crop”. International Journal of Environment and Climate Change 14 (2):374-81. https://doi.org/10.9734/ijecc/2024/v14i23952.

Downloads

Download data is not yet available.

References

Kanakala S, Ghanim M. Global genetic diversity and geographical distribution of Bemisiatabaci and its bacterial endosymbionts. PLoS ONE. 2019;14: e0213946.

Head G, Savinelli C. Adapting insect resistance management programs to local needs. Insect resistance management: Biology, economics and prediction. 2008; 305.

Sundararaj R, Amuthavalli T, Vimala D. Invasion and establishment of the solanum whitefly Aleurothrixustrachoides (Back) (Hemiptera: Aleyrodidae) in South India. Curr. Sci. 2018;115(1): 29-31.

Sundararaj R, Pushpa R. In BV. David (Ed.), The whitefly or Mealywing bugs: Bioecology, Host specificity and management. Germany: Lambert Academic Publishing. 2011;20-57.

De Barro PJ, Liu SS, Boykin LM, Dinsdale AB. Bemisiatabaci: a statement of species status. Annu. Rev. Entomol. 2011;56: 1-19.

Gosalbes MJ, Latorre A, Lamelas A, Moya A. Genomics of intracellular symbionts in insects. Int. J. Med. Microbiol. 2010;300 (5):271-278.

Amann RI, Ludwig W, Schleifer KH. Phylogenetic identification and in situ detection of individual microbial cells withoutcultivation. Microbiol. Rev. 1995; 59(1):143-169.

Takakura KI, Nishio T. Safer DNA extraction from plant tissues using sucrose buffer and glass fiber filter. Plant Res. 2012;125(6):805-807.

Marubayashi JM, Kliot A, Yuki VA, Rezende JAM, Krause-Sakate R, Pavan MA, Ghanim M. Diversity and localization of bacterial endosymbionts from whitefly species collected in Brazil. PloS One. 2014;9(9):108363.

El,Hamss H, Maruthi MN, Ally HM, Omongo CA, Wang HL, Van, Brunschot S, Colvin J, Delatte H. Spatio-temporal changes in endosymbiont diversity and composition in the African cassava whitefly, Bemisiatabaci SSA 1. Front. Microbiol. 2022;13:986226.

Li SN, Zhang C, Li F, Ren NQ, Ho SH. Recent advances of algae-bacteria consortia in aquatic remediation. Crit. Rev. Environ. Sci. Technol. 2023;53(3):315-339.

Indiragandhi P, Yoon C, Yang JO, Cho S, Sa TM, Kim GH. Microbial communities in the developmental stages of B and Q biotypes of sweetpotato whitefly, Bemisiatabaci (Hemiptera: Aleyrodidae). Appl. Biol. Chem. 2010;53(5):605-17.

Jones AG, Mason CJ, Felton GW, HooverK. Host plant and population source drive diversity of microbial gut communities in two polyphagous insects. Sci. Rep. 2019;9(1):1-11.

Saranya M, Kennedy JS, Anandham R. Functional characterization of cultivable gut bacterial communities associated with rugose spiralling whitefly Aleurodicusr-ugioperculatus Martin. 3 Biotech. 2022;12 (1):14.

Pujar K, Naik DJ, Shivanna B. Study on comparative diversity of bacterial endosymbionts in invasive rugose spiralling whitefly, Aleurodicusru-gioperculatus Martin on different hosts. Mys. J. Agricul. Sci. 2023;57(2).

Davidson EW, Rosell RC, HendrixDL. Culturable bacteria associated with the whitefly, Bemisiaargentifolii (Homoptera: Aleyrodidae). Fla. Entomol. 2000;6:159-169.

Choi MY, Ahn JH, Song J, Kim SH, Bae JW, Weon HY. Analysis of gut bacterial diversity and exploration of cellulose-degrading bacteria in xylophagous insects. Korean J. Microbiol. 2015;51(3): 209-220.

Krishnamoorthy R, ArulJose P, JanahiramanV, IndiraGandhi P, GandhiGracy R, Jalali SK, SenthilKumar M, Malathi V, Anandham R. Function and insecticidal activity of bacteria associated with papaya mealybug. Paracoccusmarginatus Williams & Granara de Willink (Hemiptera: Pseudococcidae). Biocontrol. Biocontrol. Sci. Technol. 2020; 30(8):76:2–778.