Effect of Seed Biopriming on Germination & Seedling Attributes in Chickpea (Cicer arietinum L.)

Lella Naga Swetha *

Department of Genetics and Plant Breeding, SHUATS, Prayagraj, India.

Abhinav Dayal

Department of Genetics and Plant Breeding, SHUATS, Prayagraj, India.

*Author to whom correspondence should be addressed.


The Study was conducted during Zaid season of 2022-2023 with IPC-05-59 genotype of chickpea (Cicer arietinum L.) Find out the Effect of seed biopriming on germination & seedling attributes in chickpea (Cicer arietinum L). The design of experiment was Completely Randomized Design comprising treatments [viz.T0-uninoculatedT1-Bacillus sp, T2- Mesorhizobium sp., T3- Pseudomonas sp, T4- Bacillus sp(0.5)+ Mesorhizobium sp.(0.5),T5- Pseudomonas sp.(0.5)+ Mesorhizobium sp.(0.5), T6- Bacillus sp.(0.5)+Pseudomonas sp(0.5).+ Mesorhizobium sp.(0.5), T7- Bacillus sp,T8- Mesorhizobium sp, T9- Pseudomonas sp, T10- Bacillus sp.(0.5) + Mesorhizobium sp(0.5),T11-Pseudomonas sp. (0.5)+ Mesorhizobiumsp(0.5),T12- Bacillus sp.(0.5) + Pseudomonas sp. (0.5) + Mesorhizobium sp. (0.5) . The treated seeds along with control were evaluated for their morpho-physiological, growth, yield, root parameters and biochemical parameters under laboratory conditions, The study revealed that seeds biopriming techniques with Bacillus sp.(0.5)+ Pseudomonas sp(0.5).+ Mesorhizobium sp.(0.5) concentration @ 1.0ml for 1 hours T6 recorded significantly higher germination percent (93%), rate of germination (5.38), mean germination time (11.16), root length (11.73cm),shoot length(13.27cm),seedling length(25.00cm), fresh weight (7.91gm),dry weight (1.62gm), vigor index-I (2324.91), vigor index – II (135.82), seed density (1.27), electrical conductivity (0.817), seed metabolic efficiency (1.018). which was followed by seedprimingwithT12- Bacillus sp (0.5) +Pseudomonas sp (0.5) +Mesorhizobium sp (0.5) @ 1.5ml for 2 hours and minimum was recorded in T0 (control).

Keywords: Chickpea, biopriming treatment, crbd (completely randomized block design), germinationt, speed of germination, seedling length, vigour index

How to Cite

Swetha , L. N., & Dayal , A. (2024). Effect of Seed Biopriming on Germination & Seedling Attributes in Chickpea (Cicer arietinum L.). International Journal of Environment and Climate Change, 14(2), 431–440. https://doi.org/10.9734/ijecc/2024/v14i23958


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Singh V, Upadhyay RS, Sarma BK, Singh HB. Seed bio-priming with trichoderma a sperellum effectively modulate plant growth promotion in pea. International Journal of Agriculture, Environment and Biotechnology. 2016;9(3):361- 365.

Entesari M, Sharifzadeh F, Ahmadzadeh M, Farhangfar M. Seed biopriming with trichoderma species and Pseudomonas fluorescent on growth parameters, enzymes activity and nutritional status of soybean International Journal of Agronomy and Plant Production. 2013;4(4):610-619.

Nascimento WM, Cantliffe DJ, Huber DJ. Seed aging affects ethylene production and endo-β-mannanase activity during lettuce seed germination at high temperature. Seed Science and Technology. 2005;33(1):11-17.

Rafi H, Dawar S, Zaki MJ. Seed priming with extracts of Acaciani lotica (L.) willd. ex delile and Sapindu smukorossi (L.) plant parts in the control of root rot fungi and growth of plants. Pak. J. Bot. 201547(3):1129-1135.

Kamithi KD, Wachira F, Kibe AM. Effects of different priming methods and priming durations on enzyme activities in germinating chickpea (Cicer arietinum L.). American Journal of Natural and Applied Sciences. 2016;1(1):1-9.

Abdul‐Baki A, Anderson JD. Vigor determinationin soybean seed by multiple criteria. Crop Science. 1973;13(6):630-633.

Baliyan N, Dheeman S, Maheshwari DK, Dubey RC, Vishnoi VK. Rhizobacteria isolated under field first strategy improved chickpea growth and productivity. Environmental Sustainability. 2018;1:461-469.

Chaurasia AK, Naik BP, Bara BM. Influence of priming on seed yield parameters of chickpea (Cicer arietinum L.). Journal of Pharmacognosy and Phytochemistry. 2018;7(2):753-755.

Ditta A, Imtiaz M, Mehmood S, Rizwan MS, Mubeen F, Aziz O, Qian Z, Ijaz R, Tu S. Rock phosphate-enriched organic fertilizer with phosphate-solubilizing microorganisms improves nodulation, growth, and yield of legumes. Commun. Soil Sci. Plant Anal. 2018;49(21):2715–2725.

Fahsi N, Mahdi I, Mesfioui A, Biskri L, Allaoui A. Plant growth promoting rhizobacteria isolated from the jujube (Ziziphus lotus) plant enhance wheat growth, Zn uptake, and heavy metal tolerance. Agriculture. 2021;11:316.

Gupta SC. Effect of combined inoculation on nodulation, nutrient uptake and yield of chickpea in Vertisol. Journal of the Indian Society of Soil Science. 2006;54:251-254.

Hayat R, Ali S, Amara U, Khalid R, Ahmed I. Soil beneficial bacteria and their role in plant growth promotion: A review. Annals of Microbiology. 2010;60:579-598.

Jeffries P, Gianinazzi S, Perotto S, Turnau K, Barea JM. The contribution of arbuscular mycorrhizal fungi in sustainable maintenance of plant health and soil fertility. Biology and Fertility of Soils. 2003;37:1-16.

Khan MS, Zaidi A, Wani PA. Role of phosphate solubilizing microorganisms in sustainable agriculture. A Review. Agronomy for sustainable Development. 2007;27:29- 43.

Lohitha B, Dutta A. Biopriming in chickpea (Cicer arietinum L.): An ecofriendly approach for quality seed production. Pharma Innovation. 2022;11(2):375-379.

Moeinzadeh A, Sharif-Zadeh F, Ahmadzadeh M, Tajabadi FH. Biopriming of sunflower (Helianthus annuus L.) seed with pseudomonas fluorescens for improvement of seed invigoration and seedling growth. Australian Journal of Crop Science. 2010;4(7):564-570.

Negi S, Bharat NK, Kaushal R, Rohiwala P. Screening of bioagents for seed biopriming in French bean (Phaseolus vulgaris L.) under Laboratory condition. International Journal of Communication Systems. 2020;8(3):790-793.

Pandey S, Gupta S. Evaluation of Pseudomonas sp. for its multifarious plant growth promoting potential and its ability to alleviate biotic and abiotic stress in tomato (Solanum lycopersicum) plants. Scientific Reports. 2020;10:20951.

Panse VG, Sukhatme PV. Statistical methods for agricultural workers. Indian council of agricultural research, New Delhi. 1967;381.

Pramanik K, Bera AK. Response of biofertilizer and phytohormone on growth and yield of chickpea (Cicer arietinum L.). Journal of Crop and Weed. 2012;8(2):45- 49.

Rao DG, Sinha SK. Efficiency of mobilization of seed reserves in sorghum hybrids and their parents as influenced by temperature regimes. Seed Research. 1993;2(2):97-100.

Reddy ASR, Madhavi GB, Reddy KG, Yella Reddy Gari SK, Reddy MS. Effect of seed biopriming with Trichoderma viride and Pseudomonas fluorescens in chickpea (Cicer arietinum) in Andhra Pradesh, India. In Plant growth-promoting rhizobacteria (PGPR) for sustainable agriculture, Proceedings of the 2nd Asian PGPR conference, Beijing, China. 2011;324-429.

Tambekar DH, Gulhane SR, Somkuwar DO, Ingle KB, Kanchalwar SP, Upadhye MA, Bidwai UA. Potential Rhizobium and phosphate solubilizers as a biofertilizers from saline belt of Akola and Buldhana District, India. Res. J. Agric. Biol. Sci. 2009;5(4):578-582.

Tanwar A, Aggarwal A, Kaushish S, Chauhan S. Interactive effect ofAM fungi with Trichoderma virideand Pseudomonas fluorescens on growth and yield of broccoli. Plant Protection Science. 2013;49(3):137-145.

Valverde A, Burgos A, Fiscella T, Rivas R, Igual JM. Differential effects of coinoculations with Pseudomonas jessenii PS06 (aphosphate–solubilizingbacterium) and Mesorhizobium ciceri C–2/2 strains on the growth and seed yield of chickpea under green house and field conditions. Plant and Soil. 2006;287:43-50.

Van der Maesen LJG. Origin, history and taxonomy of chickpea. In The Chickpea. 1987;11- 34.

Veena Shri Jainapur, Shalini N. Huilgol SM. Vastrad and Jolli RB. Biopriming of chickpea seeds with biocontrol agents for enhanced seedling vigour and reduced seed borne diseases. International Journal of Current Microbiology and Applied Sciences. 7(2): 2746-2750.

Vishwas S, Chaurasia AK, Bara BM, Debnath A, Parihar NN, Brunda K, Saxena R. Effect of priming on germination and seedling establishment of chickpea (Cicer arietinum L.) seeds. Journal of Pharmacognosy and Phytochemistry. 2017;6(4):72-74.

Vishwas S, Chaurasia AK, Bara BM, Debnath A, Parihar NN, Brunda K, Saxena R. Effect of priming on germination and seedling establishment of chickpea (Cicer arietinum L.) seeds. Journal of Pharmacognosy and Phytochemistry. 2017;6(4):72- 74.

Yadav SK, Dave A, Sarkar A, Singh HB, Sarma BK. Co-inoculated biopriming with Trichoderma, Pseudomonas and Rhizobium improves crop growth in Cicer arietinum and Phaseolus vulgaris. International Journal of Agriculture. Environment and Biotechnology. 2013;6: 255-259.