Influence of Different Culture Media on Growth and Sporulation of Colletotrichum capsici

Ranjana Joshi *

Department of Plant Pathology, University of Agricultural Sciences, Raichur, Karnataka, 584104, India.

Yenjerappa, S. T.

Department of Plant Pathology, University of Agricultural Sciences, Raichur, Karnataka, 584104, India.

Aswathanarayana, D. S.

Department of Plant Pathology, University of Agricultural Sciences, Raichur, Karnataka, 584104, India.

Sreedevi S Chavan

Department of Plant Pathology, University of Agricultural Sciences, Raichur, Karnataka, 584104, India.

Kisan, B.

Department of Molecular Biology and Agriculture Biotechnology, University of Agricultural Sciences, Raichur, Karnataka, 584104, India.

Lakshmikant, M.

Department of Soil Science and Agriculture Chemistry (Biochemistry), University of Agricultural Sciences, Raichur, Karnataka, India.

*Author to whom correspondence should be addressed.


Abstract

Aim: To know the best culture media for growth and development of Colletotrichum capsici causing chilli anthracnose.

Study Design: Twelve different culture media were selected and three replications were maintained.

Place and Duration of Study: Department of Plant Pathology, University of Agricultural Sciences, Raichur, Karnatka, which was carried out in the year 2021-2023.

Methodology: The laboratory study was conducted to know the best nutrient media for the growth and sporulation of the pathogen. The pathogen was isolated from infected chilli fruit using tissue isolation technique and pure culture was maintained. Twelve different nutrient media were selected and tested for the growth and sporulation of the pathogen in three replications.

Results: Among twelve media tested, Red chilli dextrose agar have promoted the maximum growth of C. capsici (90.0 mm), which was followed by carrot dextrose agar and potato carrot agar (89.50 mm). Green chilli extract agar significantly promoted minimum growth of C. capsici (33.50 mm), which was followed by red chilli extract agar (48.00 mm) and Sarbouraud’s agar (48.50 mm). The cultural characters were varied among the media.

Conclusion: Potato dextrose agar and carrot dextrose agar media were proved to be best media for growth and sporulation of Colletotrichum capsica.

Keywords: Chilli anthracnose, Colletotrichum capsici, culture media, growth, sporulation


How to Cite

Joshi , R., Yenjerappa, S. T., Aswathanarayana, D. S., Chavan , S. S., Kisan, B., & Lakshmikant, M. (2024). Influence of Different Culture Media on Growth and Sporulation of Colletotrichum capsici. International Journal of Environment and Climate Change, 14(2), 555–561. https://doi.org/10.9734/ijecc/2024/v14i23969

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