Two Prominent Entomopathogenic Fungi Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae (Metchnikoff) Sorokin as Natural Enemies of Lepidopteran Larvae from Rayalaseema Region of Andhra Pradesh

S. Abdul Mujeeb *

Department of Entomology, S.V. Agricultural College, Tirupati-517502, (Acharya N.G. Ranga Agricultural University, Andhra Pradesh), India.

K. Manjula

Department of Entomology, S.V. Agricultural College, Tirupati-517502, (Acharya N.G. Ranga Agricultural University, Andhra Pradesh), India.

P. N. Harathi

Department of Entomology, IFT, RARS, Tirupati-517502, Andhra Pradesh, India.

A. Kandan

Department of Plant Pathology, ICAR-NBAIR, Bengaluru – 560024, India.

P. Lavanya Kumari

Department of Statistics and Computer Applications, S.V. Agricultural College, Tirupati-517502, (Acharya N.G. Ranga Agricultural University, Andhra Pradesh), India.

*Author to whom correspondence should be addressed.


Roving survey was conducted for searching the natural occurrence of Beauveria bassiana and Metarhizium anisopliae during kharif season of 2022 and rabi season of 2022-23 in Kurnool, Kadapa, Chittoor and Anantapuramu districts of Andhra pradesh. The crops surveyed were Groundnut, Maize, Cotton, greengram and blackgram during September to October of kharif season and January to February of rabi season. The pest population found in among groundnut crops were Spodoptera litura, Helicoverpa armigera and Aproarema modicella and maize, Spodoptera frugiperda which were found at vegetative stage. The fungal infected cadavers of lepidopteran lavae were observed on the soil below the canopy or adhering to the foliage of groundnut and maize crops. The larval cadavers were collected in separate aseptic vials and soil samples were also collected from the surveyed fields. During the survey, it was found that the mean number of infected cadavers with fungi varied from 2.6 to 3.2 per square meter in Kurnool district during Sep-Oct of 2022. In Anantapuramu district the mean number of cadavers were 1.4-2.2 during Jan-Feb of 2023. In Chittoor district the mean number of infected cadavers were varied from 2.4-3.6, whereas in Kadapa district it was 0.2 per square meter during Jan-Feb of 2023. The cadavers were cultured in the lab and based on morphological characters confirmed the isolates as Beauveria bassiana and Metarhizium anispoliae. The cadavers of B. bassiana were found to be high in Kurnool district followed by Chittoor district and least in Kadapa district where as the cadavers of M.anispoliae were found to be higher in Chittoor district followed by Anantapuramu district and Nil in Kadapa district.

Keywords: Survey, occurrence, entomopathogenic fungi, Beauveria bassiana, Metarhizium anisopliae

How to Cite

Mujeeb, S. Abdul, K. Manjula, P. N. Harathi, A. Kandan, and P. Lavanya Kumari. 2024. “Two Prominent Entomopathogenic Fungi Beauveria Bassiana (Balsamo) Vuillemin and Metarhizium Anisopliae (Metchnikoff) Sorokin As Natural Enemies of Lepidopteran Larvae from Rayalaseema Region of Andhra Pradesh”. International Journal of Environment and Climate Change 14 (6):105-14.


Download data is not yet available.


Hajek AE. Ecology of terrestrial fungal entomopathogens. Advances in Microbial Ecology. 1997;15:193-249.

Inglis GD, Goettel MS, Butt TM, Strasser H. Use of hyphomycetous fungi for managing insect pests. Fungi as Biocontrol Agents. Progress, Problems and Potential. CABI Publishing. 2001;23- 69.

Charnley AK. Mycoinsecticides: Present use and future prospects. In: Progress and Prospects in Insect Contour. BCPC Monograph. 1989;43:165-181.

Ferron P. Fungal control In comprehensive insect physiology, biochemistry and pharmacology. Pergamon Press, New York. 1985;12:313-346.

Samson RA, Evans HC, Latge JP. Atlas of Entomopathogenic Fungi. Springer-Verlag, New York.1988;187.

Ambethgar V, Loganathan M. Incidence of green muscardine fungus, Nomuraea rileyi (Farlow) Samson on Spodoptera litura (Fab.) in soybean, Glycine max (L.) Merrill from Tamil Nadu (India). Journal of Entomological Research. 1998;22:195- 196.

Azizoglu U, Karaborklu, S, Fıratlıgil- Durmuş E. Metarhizium spp. and their potential for controlling insect pests. Turkish Journal of Entomology. 2020; 44(2):123-135.

Bohara RL, Sharma S, Khatri S. Efficacy of commercial and indigenous strains of Metarhizium anisopliae against white grubs. Journal of Entomology and Zoology Studies. 2018;6(3): 805-810.

Rijal JP. Efficacy of Metarhizium anisopliae against Helicoverpa armigera in chickpeas. Journal of Biocontrol Science and Technology. 2018;28(3): 256-267.

Peeru Saheb Y, Manjula K, Devaki K, Sarada Jaya Lakshmi R, Ravindra Reddy B, Venkateswarlu NC. Nomuraea rileyi (Farlow) Samson Incidence on Spodoptera litura and Spodoptera frugiperda in Andhra Pradesh. Int. J. Curr. Microbiol. App. Sci. 2021;10(02): 1262-1270.

Nidhi K, Gaur N, Pandey R. A survey of the local isolates of entomopathogenic fungi in Uttarakhand region. Journal of Entomology and Zoology Studies. 2018;6(1):1725- 1730.

Dhar S, Jindal V, Jariyal M, Gupta VK. Molecular characterization of new isolates of the entomopathogenic fungus Beauveria bassiana and their efficacy against the tobacco caterpillar, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). Egyptian Journal of Biological Pest Control. 2019;29:8.

Abarna VP, Vishnupriya R. DNA barcoding reveals the natural occurrence of Beauveria bassiana (Balsamo) Vuillemin in two-spotted spider mite, Tetranychus urticae Koch in Bhendi [Abelmoschus esculentus (L.) Moench] ecosystem in Coimbatore district of Tamil Nadu. Egyptian Journal of Biolological Pest Control. 2022;32:128.

Cokola MC, Ben Fekih I, Bisimwa EB. Natural occurrence of Beauveria bassiana (Ascomycota: Hypocreales) infecting Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) and earwig in eastern DR Congo. Egyptian Journal of Biological Pest Control. 2023;33:54

Manjula K, Saheb PY, Sudheer JM, Rao RA. First record on occurrence of epizootics of Metarhizium anisopliae (Metchnikoff) sorokin on groundnut leaf miner, Aproarema modicella (Deventer). International Journal of Current Research. 2019;11(4):2715- 2717.

Velavan V, Rangeshwaran R, Sivakumar G. Occurrence of Metarhizium spp. isolated from forest samples in South India and their potential in biological control of banana stem weevil Odoiporus longicollis Oliver. Egyptian Journal of Biological Pest Control. 2021;31:131.

Ayele BA, Muleta D, Venegas J, Assefa F. Morphological, molecular, and pathogenicity characteristics of the native isolates of Metarhizium anisopliae against the tomato leaf miner, Tuta absoluta (Meyrick 1917) (Lepidoptera: Gelechiidae) in Ethiopia. Egyptian Journal of Biological Pest Control. 2020;30:59-65.

Elham MSH, Pong KK, Lee GEL, Azmi WA. Occurrence of entomopathogenic fungus, Metarhizium anisopliae isolated from Island, BRIS and coastal soils of Terengganu, Malaysia. Journal of Sustainability Science and Management. 2018;13(5): 179-189.

Korosi GA, Wilson BAL, Powell KS, Ash GJ, Reineke A, Savocchia S. Occurrence and diversity of entomopathogenic fungi (Beauveria spp. and Metarhizium spp.) in Australian vineyard soils. Journal of Invertebrate Pathology. 2019;4(5):16-23.

Freed S, Saleem MA, Khan MB, Naeem B. Prevalence and effectiveness of Metarhizium anisopliae against Spodoptera exigua (Lepidoptera: Noctuidae) in Southern Punjab, Pakistan. Pakistan Journal of Zoology. 2012; 44(3):753-758.

Mamatha M, Hosamani A, Prashanth G, Bhuvaneshwari, Hanchinal SG, Ghante VN, Aswathanarayana DS. Morphological characterization of native isolates of hypocreale fungi Metarhizium rileyi (Farl.) Samson from Maize Fall Armyworm. Journal of 8. Advances in Biology & Biotechnology. 2024;27(6):36–43. Available:

Hussain SB, Aslam M, Aslam A, Noor S, Zubair M. Production of copper nanoparticles by Aspergillus niger and evaluation of their biomedical applications. Asian Journal of Biochemistry, Genetics and Molecular Biology. 2023;15(2): 46–59. Available: 023/v15i2332

Shah PA, Pell JK. Entomopathogenic fungi as biological control agents. Applied microbiology and biotechnology. 2003 Jun;61(5):413-23.